Sequence analysis of HBV in primary hepatomas patients infected with HBV
10.3760/cma.j.issn.1003-9279.2017.02.002
- VernacularTitle: 原发性肝癌合并HBV感染者的HBV基因特征分析
- Author:
Shaobin DONG
1
;
Fuzhen WANG
2
;
Shuang ZHANG
1
;
Guomin ZHANG
2
;
Feng WANG
1
;
Qingling MENG
1
;
Liping SHEN
1
;
Shengli BI
1
Author Information
1. Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
2. National Immunization Programme, Chinese Center for Disease Control and Prevention, Beijing 100052, China
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Hepatitis B virus;
Risk factor;
Sequence characteristics
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(2):92-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the relationship between the development of hepatocellular carcinoma(HCC) and HBV gene characteristics among the HCC patients with hepatitis B virus (HBV) infection.
Methods:Some acute and chronic hepatitis B patients were collected as control group and HBV associated HCC patients as HCC group. Serum samples of subjects were tested for HBV serological markers. HBV DNA of those samples had been extracted and nested PCR was used to amplify the sequence of HBV DNA. Furthermore, MEGA 6.0 and Bioedit softwares were used to made phylogenetic trees and analyze the gene mutations.
Results:The sequences of S region and BCP/Precore region of HBV were amplified from 86 samples in study group and 39 samples in control group. The prevalence of PreS deletion, A1762T and A1762T/G1764A in HCC group were 39.53%, 74.42% and 72.09% respectively, and in control group were 20.51%, 53.85% and 53.85% respectively. The statistical differences of them were significant. The prevalence of A1762T and A1762T/G1764A in ≥ 50 years group were higher than that of < 50 years group. The prevalence of A1762T, G1764A and A1762T/G1764A of subjects who infected genotype C were higher than those infected genotype B. On the contrary, the prevalence of G1896A of subjects who infected genotype C were lower than that of genotype B. It was found that ≥ 50 years, genotype C and G1896A mutation were independently associated with HCC. The risk for suffer from HCC of ≥50 years group, genotype C group and G1896A group were 9.349, 28.875 and 7.648 times compared with < 50 years group genotype B group and without G1896A mutation group, respectively.
Conclusions:The population of ≥50 years or genotype C had a higher prevalence of A1762T, A1762T/G1764A, ≥50years、genotype C、G1896A were independently associated with HCC, as compared with the subjects of the control group.
