Effects of bisphenol A on the expression of N-cadherin, Vimentin and FSHR in rat Sertoli cells
10.3760/cma.j.issn.1001-9391.2017.02.005
- VernacularTitle: 双酚A对大鼠睾丸支持细胞N-钙黏蛋白和波形蛋白及卵泡刺激素受体表达的影响
- Author:
Wei CHEN
1
;
Peng DUAN
;
Sha TANG
;
Wenting HUANG
;
Chao QUAN
;
Suqin QI
;
Kedi YANG
Author Information
1. MOE Key Laboratory of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
- Publication Type:Journal Article
- Keywords:
Bisphenol A;
Sertoli cells;
N-cadherin;
Vimentin;
FSHR
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(2):101-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of BPA on the expression of N-cadherin, Vimentin and FSHR in rat Sertoli cells.
Methods:Primary Sertoli cells collected from prepuberty rats (18-21 d) were cultured for 48 h, and then they were treated with 0, 30, 50, 70 μmol/L BPA respectively for 24 h. The methods of MTT, real-time quantitative PCR and Western blotting were utilized to measure the cell ability of Sertoli cells, the mRNA and protein expression levels of N-cadherin, Vimentin and FSHR respectively.
Results:Compared with control, the cell abilities of Sertoli cells in 50 μmol/L BPA group and 70 μmol/L BPA group increased significantly (P<0.05) . The cell abilities of Sertoli cells decreased with the increases of exposure doses of BPA. Compared with control, the expression of N-cadherin mRNA only increased in 30 μmol/L BPA group (P<0.05) , the expression of Vimentin mRNA decreased significantly in all doses group of BPA (P<0.05) , the expression of FSHR mRNA increased in all doses group of BPA (P<0.05) . Compared with the control, the protein levels of N-cadherin increased significantly in 50 μmol/L BPA group (P<0.05) , the protein levels of Vimentin decreased significantly in all doses group of BPA (P<0.05) , the protein levels of FSHR decreased significantly in 50 μmol/L BPA group and 70 μmol/L BPA group (P<0.05) .
Conclusion:The mechanism of testicular toxicity from BPA might be the alterations of N-cadherin, Vimentin and FSHR by disturbing normal spermatogenesis.
