Clinical value of a rapid respiratory syncytial virus antigen detection in point-of-care testing
10.3760/cma.j.issn.0578-1310.2017.02.017
- VernacularTitle: 人呼吸道合胞病毒快速抗原检测方法的床旁检测适用性探讨
- Author:
Yaxin DING
1
;
Run TIAN
;
Yuan QIAN
;
Yu SUN
;
Jie DENG
;
Fang WANG
;
Runan ZHU
;
Linqing ZHAO
Author Information
1. Laboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing 100020, China
- Publication Type:Clinical Trail
- Keywords:
Respiratory syncytial virus;
Point-of-care testing;
Antigen detection
- From:
Chinese Journal of Pediatrics
2017;55(2):139-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the clinical value of a rapid respiratory syncytial virus (RSV) antigen detection in point-of-care testing (POCT).
Method:A total of 209 specimens, including 78 throat swabs (TS) and 131 nasopharyngeal aspirates (NPAs), were collected from inpatients who visited the Children′s Hospital Affiliated to the Capital Institute of Pediatrics and were diagnosed as acute respiratory infection from 5 January to 7 February, 2015. These specimens were tested for RSV by a rapid antigen detection kit which was compared with reverse transcription polymerase chain reaction (RT-PCR) and direct immunofluorescence assay (DFA) for RSV detection.
Result:Compared with DFA for NPAs, the sensitivity and specificity of rapid antigen detection were 83.9% and 97.3%, respectively, with Kappa value of 0.86; Compared with RT-PCR, the sensitivity (NPAs, 74.2%; TS, 77.8%) and specificity (NPAs, 100.0%; TS, 92.0%) of rapid antigen detection were high, too, with Kappa value of 0.74 in NPAs and 0.62 in TS. However, the RSV positive rate of rapid antigen detection in TS (21.7%) from pediatric patients with acute lower respiratory tract infection was lower than that in NPAs (78.3%), as well as that of RT-PCR (7.3% in TS verse 78% in NPAs). The RSV rapid antigen detection kit can be finished in about 10 minutes.
Conclusion:With characteristics of high specificity, high sensitivity, being rapid, efficient and easy to operate in comparison with DFA and RT-PCR, RSV rapid antigen detection in this study is suitable for POCT. For pediatric patients with acute respiratory tract infection, NPA was better than TS for RSV detection.
