Serotype identification and antibiotic susceptibility of Shiga toxin-producing Escherichia coli in the Weishan area in Shandong Province, China
10.3760/cma.j.issn.0253-9624.2017.01.014
- VernacularTitle: 山东省微山县动物粪便中产志贺毒素大肠埃希菌血清型鉴定及耐药性分析
- Author:
Chunchun SHAO
1
;
Bin HU
;
Zhenwang BI
;
Zengqiang KOU
;
Ming FANG
;
Baoli CHEN
;
Zhenqiang BI
Author Information
1. Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan 250012, China
- Publication Type:Journal Article
- Keywords:
Shiga toxin-producing Escherichia coli;
Serotyping;
Drug Resistance
- From:
Chinese Journal of Preventive Medicine
2017;51(1):70-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the serotypes and drug resistance profiles of Shiga toxin-producing Escherichia coli (STEC) in animal stools from the Weishan area in Shandong Province, China. To provide the basis for further study.
Methods:Five hundred animal stool samples (from pigs, cattle, sheep, dogs and birds) were collected from the Weishan area and STEC strains were isolated from these samples. Strains were serotyped by a serum agglutination test, and their drug resistance profiles were determined through antimicrobial sensitivity experiments. In this study, PCR was used to detect tetracycline resistance genes (tetA, tetB, tetC, tetD) and beta-lactam resistance genes (blaSHV-1, blaCTX-M, blaTEM).
Results:Sixteen strains of STEC were isolated from animal stool samples. Thirteen strains were isolated from pig stool samples, two from bovine stool samples and one from a sheep stool sample. Two of the strains were identified as E. coli O157:H7, and other 14 strains were non-O157 STEC of different serotypes. Antimicrobial sensitivity experiments showed that 15 of the strains were multidrug resistant. The rates of resistance were as follows: nalidixic acid (12/16 strains), sulfisoxazole (11/16), trimethoprim and sulphame-thoxazole (11/16), doxycycline (9/16), azithromycin (9/16), tetracycline (9/16), chloramphenicol (8/16) and streptomycin (8/16). Therefore, nalidixic acid showed the highest rate of resistance among the strains, followed by trimethoprim and sulphame-thoxazole, and sulfisoxazole. Resistance to cefepime or imipenem was not detected. In total, three types of drug resistance genes (tetA, tetB and tetC) were detected among the 16 strains.
Conclusion:The results showed that STEC strains isolated from animals in the Weishan area were of a range of serotypes. The 16 strains of STEC isolated from animal stools in this area were resistant to a number of antibiotics, with many strains displaying multidrug resistance.
