Clinical significance of expressions of EVI1 and BRE genes in 47 acute leukemia patients with MLL rearrangement
10.3760/cma.j.issn.0253-2727.2017.01.005
- VernacularTitle: 47例MLL重排急性白血病患者EVI1和BRE基因表达及其临床意义
- Author:
Yanlei GONG
1
;
Jingren ZHANG
;
Jun ZHANG
;
Shuxiao BAI
;
Jiannong CEN
;
Hongjie SHEN
;
Huiying CHOU
;
Suning CHEN
;
Jinlan PAN
Author Information
1. Department of Hematology, The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Homeostasis, Suzhou 215006, China
- Publication Type:Journal Article
- Keywords:
Leukemia, acute;
Gene rearrangement, MLL;
In situ hybridization, fluorescence;
Gene, EVI1;
Gene, BRE
- From:
Chinese Journal of Hematology
2017;38(1):22-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the overexpression frequencies of BRE and EVI1, the correlation between BRE and EVI1 expressions and their possible clinical implications in 11q23/MLL rearrangement acute leukemia.
Methods:Cytogenetic examination of bone marrow cells was performed by short-term culture method. R-banding technique was used for karyotype analysis. 47 patients were detected by interphase fluorescence in situ hybridization (FISH) with dual-color break apart MLL probe. The expressions of EVI1 and BRE genes were detected by real time quantitative reverse transcription polymerase chain reaction (RQ-PCR) . The correlation and prognostic significance were statistically tested.
Results:11q23/MLL rearrangements were confirmed by karyotyping and FISH, respectively in 47 patients. According to immunophenotypic analyses of 37 patients, 5 patients showed positive for CD19, CD79a or CD10, 1 for CD7; the others for CD33, CD13, CD14 and CD15, and 16 of them for CD34. Of the 47 patients, 18 patients showed EVI1 overexpression and most of them presented with t (6;11) and M4/M5. The EVI1 expression was high in t (6;11) or t (9;11) subgroup comparable with levels observed in normal subgroup (P=0.038, 0.022, respectively) . 15 patients showed high BRE expression, and most of them presented with t (9;11) and M4/M5. High BRE expression was found in t (4;11) , t (6;11) , t (9;11) and t (11;19) subgroups, respectively by comparing with normal subgroup. The BRE expression was higher in t (4;11) (P=0.004) or t (9;11) (P=0.012) subgroup than in t (6;11) subgroup. Patients with EVI1 overexpression had a short survival compared with those with low EVI1 expression (P=0.049) and it also did in t (9;11) subgroup (P=0.024) . Patients with t (9;11) and high BRE expression had a long survival compared with those with t (9;11) and low BRE expression (P=0.024) .
Conclusion:The EVI1 overexpression was significantly frequent in acute leukemia patients with 11q23/MLL rearranged, especially within t (6;11) subgroup and M4/M5, which was associated with an inferior outcome. High BRE expression was observed frequently in 11q23/MLL-rearranged acute leukemia especially within t (9;11) subgroup and M5.
