Establishment and characterization of a laryngeal squamous cell carcinoma cell line
10.3760/cma.j.issn.1673-0860.2017.01.008
- VernacularTitle: 喉鳞状细胞癌细胞系的建立及其生物学特性研究
- Author:
Ru WANG
1
,
2
;
Xiaocui BIAN
3
;
Yuqin LIU
3
;
Jugao FANG
2
,
4
,
5
;
Hong WANG
6
;
Honggang LIU
4
;
Meng LIAN
5
,
6
;
Hongzhi MA
5
,
6
;
Ling FENG
5
,
6
;
Haizhou WANG
5
,
6
Author Information
1. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China
2. Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, China
3. Institute of Basic Medical Science, Chinese Academy of Medical Sciences, Beijing 100005, China
4. Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing 100730, China
5. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China
6. Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, China
- Publication Type:Journal Article
- Keywords:
Laryngeal neoplasms;
Tumor cells, cultured;
Carcinoma, squamous cell;
Cell line
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2017;52(1):44-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a laryngeal squamous cell carcinoma (LSCC) cell line through primary cell culture and observe its biological characteristics.
Methods:Tissue block culture method was used for primary cell culture. After LSCC cells passed 25 times in vitro, the morphology of cells was observed, keratin was stained histochemically, cell cycle was tested by PI-FACS, and the specie of cells was detected by PCR and short tandem repeat(STR) typing.
Results:This newly established LSCC cell line was named as TR-LCC-1, most of the cancer cells were polygonal shape, like the cobblestone, loss of contact inhibition and with overlapping growth. Cell size was large and cell pleomorphism was very obvious. Cytokeratin staining was positive. After 6 months of continuous culture in vitro, the TR-LCC-1 cells passed more than 30 times, and cell doubling time was 201.2h. Cell cycle assay indicated that G1 phase accounted for 51.71%, S phase was 44.56%, and G2 phase was 2.28%. Mycoplasma test showed no mycoplasma contamination. Cell species identification identified TR-LCC-1 was human-derived cells. STR detection showed P26 and P6 were same, and they were different from the STR typing of disclosed cells.
Conclusion:The establish ment of the new laryngeal squamous carcinoma cell line TR-LCC-1 can be helpful to the research for laryngeal squamous cell cancer.
