The effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts
10.3760/cma.j.issn.1009-4598.2017.01.012
- VernacularTitle: 干扰人整合素蛋白β5基因表达对瘢痕疙瘩成纤维细胞增殖能力的影响
- Author:
Tongtong YAN
1
;
Minliang CHEN
;
Kui MA
;
Xiaobing FU
Author Information
1. Beijing Friendship Hospital, Beijing 100050, China
- Publication Type:Journal Article
- Keywords:
β
5-integrin;
Keloid;
Fibroblast;
Proliferation
- From:
Chinese Journal of Plastic Surgery
2017;33(1):49-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of down-regulated ITGB5 expression on the proliferation of keloid fibroblasts and clarify the possible role of β5-integrin(ITGB5) in keloid.
Methods:Construct lentiviral sh-RNA-expression vector targeting ITGB5 and infect keloid fibroblasts, the expression of ITGB5 were detected by Western Blot, the proliferation ability was identified by MTT.
Results:The expression quantity of ITGB5 mRNA and protein in KFb group, LV-NC group and LV-KFb group are 1.00±0.00, 1.08±0.05, 0.34±0.01 and 0.91±0.03, 0.93±0.02, 0.28±0.07. Compared with LV-NC group and KFb group, the expression quantity of ITGB5 mRNA and protein in LV-KFb group decreased significantly(P<0.01). Compared with LV-NC group and KFb group, the proliferation rate decreased significantly in LV-KFb group at 48 h(P<0.01).
Conclusions:These results suggest that ITGB5 can accelerate fibroblasts proliferation in keloids.
