Pyrroloquinoline quinone inhibited oxidative stress induced-apoptosis of Schwann cells via mitochondrial apoptotic signaling pathway in vitro
10.3760/cma.j.issn.1009-4598.2017.01.011
- VernacularTitle: 线粒体途径在吡咯喹啉醌抑制氧化应激诱导施万细胞凋亡中的作用
- Author:
Bin HE
1
;
Haiying TAO
;
Ailin WEI
;
Xiaohai LI
;
Haohuan LI
Author Information
1. Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan 430060, China
- Publication Type:Journal Article
- Keywords:
Pyrroloquinoline quinone;
Schwann cell;
Oxidative stress;
Apoptosis;
Mitochondria
- From:
Chinese Journal of Plastic Surgery
2017;33(1):43-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanisms of Pyrroloquinoline quinone (PQQ) against oxidative stress induced apoptosis in Schwann cells (SCs).
Methods:SCs were cultured in vitro, identified by S-100 immunofluorence staining. SCs were divided into control group, H2O2 induced group, H2O2 + PQQ treated group. CCK-8 assay was used to detect cell proliferation. Apoptosis was detected by flow cytometry with Annecin V-FITC/PI staining, mitochondrial transmembrane potential was detected by flow cytometry with JC-1 labeled staining, cytochrome C (CytC), Bax and Caspase-9 protein levels was detected by Western blot analysis.
Results:In this study, the S-100 positive cells were more than 95%, cell proliferation was decreased in H2O2 induced SCs, apoptotic rate was increased, mitochondrial transmembrane potential was decreased, CytC, Bax and Caspase-9 protein levels were increased. After PQQ added, cell proliferation was increased, apoptotic rate decreased, mitochondrial transmembrane potential increased, CytC, Bax and Caspase-9 protein levels decreased.
Conclusions:PQQ protects SCs from oxidative induced apoptosis by inhibiting mitochondrial signaling pathway.
