Effects of PRX-2 gene on the phenotype changes of epidermal stem cells differentiating into sweat gland cells
10.3760/cma.j.issn.1009-4598.2017.01.010
- VernacularTitle: 氧还蛋白过氧化物2在表皮干细胞向汗腺细胞诱导分化过程中对表型改变的影响
- Author:
Fuhuan CHEN
1
;
Huifeng SONG
;
Ximin GUO
;
Xiaotong YUE
;
Lingying LIU
;
Yong ZHOU
;
Donghua LIU
;
Zhiyong QIAN
;
Tongmin WANG
;
Xiuye HE
Author Information
1. Department of Burn and Plastic Surgery, Burns Institute of PLA, First Affiliated Hospital of PLA General Hospital, Beijing 100048, China
- Publication Type:Journal Article
- Keywords:
Epidermal Stem cells;
Sweat gland cells;
Differentiation;
PRX-2
- From:
Chinese Journal of Plastic Surgery
2017;33(1):37-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of PRX-2 gene on phenotype changes in epidermal stem cells differentiating into sweat gland cells.
Methods:Epidermal stem cells and sweat gland cells separated and cultured from healthy foreskin and adult full-thick skin respectively, were identified by immunofluorescence staining. Lentiviral vector-mediated overexpression and knockdown of PRX-2 gene in epidermal stem cells were performed respectively, with empty vector-mediated epidermal stem cells as a control group. Overexpression、blank control and knowdown group′s PRX-2 expressions in gene and protein levels were detected using RT-PCR and Western blot technology. The ESCs of each group were co-cultured with sweat gland cells through transwell plate, and the expressions of CEA and β1 integrin in epidermal stem cells were determined by flow cytometry before and after co-culturing.
Results:Epidermal stem cells and sweat gland cells were in line with their respective specific antigens. Before co-cultured, epidermal stem cells highly expressed β1 integrin (98.69±0.67)%, hardly expressed CEA (6.20±3.15)%. After co-cultured, β1 integrin expression levels were showed as knockdown group (19.30±0.53)% blank control group (51.20±0.79)%> overexpress group (45.91±0.93)%. There had significant differences between those of each two groups.
Conclusions:PRX-2 gene can inhibit the phenotypic change of Epidermal Stem Cells differentiating into Sweat Gland Cells and improve the ability to maintain their own specific antigens.
