Generation and preliminary characterization of monoclonal antibodies against neuraminidase of H7N9 subtype influenza A virus
10.3760/cma.j.issn.1003-9279.2017.01.014
- VernacularTitle: 抗H7N9亚型流感病毒神经氨酸酶单克隆抗体的制备及初步鉴定
- Author:
Yueyang YU
;
Yiran XIE
;
Yingzhu CHEN
1
,
2
;
Ying SUN
;
Heng ZHANG
;
Jian LU
;
Liqi LIU
;
Dayan WANG
;
Yuelong SHU
;
Kun QIN
;
Jianfang ZHOU
Author Information
1. Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
2. Wenzhou Medical University, Wenzhou 325035, China
- Publication Type:Journal Article
- Keywords:
Influenza virus;
Neuraminidase;
Monoclonal antibody
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(1):66-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop the monoclonal antibody (mAb) against neuraminidase of H7N9 subtype influenza A virus and identify its biological function.
Methods:Female 8 week-old BALB/c mice were immunized and the splenocytes of the mice were fused with Sp2/0 myeloma cells. Indirect ELISA was used to screen hybridoma and the positive clones were subject to be subcloned. Positive clones were identified and the monoclonal antibodies(mAbs) were obtained by purifying the ascetic fluid of mice injected with the hybridoma. The NA-binding as well as neuraminidase-inhibition activity of these mAbs were determined.
Results:Three mAbs against neuraminidase of H7N9 subtype influenza A virus, 1G8, 3C4 and 4E8, were obtained. They demonstrated different epitop-recognizing. 3C4 and 4E8 exhibited neuraminidase inhibitory activity, with a IC50 of 1.45 μg/ml and 8.65 μg/ml, respectively.
Conclusions:The results suggested that mAbs specific to neuraminidase of H7N9 subtype influenza A virus were developed, providing an useful tool in control and preventing the novel H7N9 influenza A virus.
