Analysis of HA1 gene of influenza A H1N1 pdm09 virus from a clustered human cases
10.3760/cma.j.issn.1003-9279.2017.01.009
- VernacularTitle: 聚集性感染甲型H1N1流感病毒的HA1基因特征分析
- Author:
Pengfei YANG
1
;
Qingli YAN
1
;
Xuezheng MA
2
;
Liping ZHANG
2
;
Wei ZHEN
3
;
Chuncheng LIU
1
;
Yadong XING
1
;
Haibo YAO
1
;
Nanjiang HE
1
;
Kongxin HU
2
Author Information
1. Huai’an Center for Disease Control and Prevention, Huai’an 223001, China
2. Chinese Academy of Inspection and Quarantine, Institute of Health Quarantine, 100123 Beijing, China
3. National Institute for Viral Disease Control and Prevention, China CDC, 102206 Beijing, China
- Publication Type:Journal Article
- Keywords:
A clustered case;
Influenza A(H1N1)pdm09 virus;
HA1 gene;
Phylogenetic analysis;
Amino acid substitution
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(1):42-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the viral etiology of a clustered case of human infection outbreak in the middle school of Huai’an city.
Methods:Nasopharyngeal swab samples from patients were collected and rapidly detected by Real-time RT-PCR and the target virus isolated in cells. Furthermore, HA1 segments of target virus were amplified by RT-PCR and sequenced. The genetic and phylogenetic analysis based on HA1 genes was computed.
Results:Influenza A(H1N1)pdm09 viral nucleic acid in 11 nasopharyngeal swab samples from patients in the outbreak were positive. Compared to the vaccine strains A/California/07/2009, the Huai’an isolates, nucleotide identity was 97.7%-98.1%, and amino acid identity was 96.6%-97.4%. Phylogenetic analysis of HA1 segment sequences indicated that the Huai’an strains from the outbreak were related closely to the viruses isolated in the year of 2014. Sequence analysis indicated that the Huai’an isolates had no amino acid substitution in the receptor binding sites and glycosylation sites, while in the Ca1 of antigenic determinant of HA1 the Huai’an isolates had an amino acid substitution of S for T at 220.
Conclusions:The pathogen of the clustered case of human infection was Influenza A(H1N1)pdm09 virus. Though the Huai’an isolates had one animo acid substitution in the Ca1 of antigenic determinant, the antigenicity characteristic remained unchanged.
