Typing analysis and detection of HPV for condyloma acuminata tissues of perianal
10.3760/cma.j.issn.1003-9279.2017.01.007
- VernacularTitle: 肛周尖锐湿疣组织HPV检测与型别分析
- Author:
Zhaojie XU
1
;
Yewei DENG
1
;
Rui LI
2
Author Information
1. Department of Colorectal, Zhengzhou People＇s Hospital Affiliated to Southern Medical University, Zhengzhou 450000, China
2. Clinical Laboratory, Zhengzhou Children＇s Hospital, Zhengzhou 450053, China
- Publication Type:Journal Article
- Keywords:
Papillomavirus human;
Genotyping;
Condyloma Acuminata
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(1):35-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To learn the prevalence of HPV infection and its genotypes distribution in perianal warts, discuss the association between HPV infection and age and gender, and assess the methodology performance of PCR-reverse dot blot(PCR-RDB) to detect HPV.
Methods:HPV genotypes were detected in 316 paraffin specimens of confirmed or suspected perianal condyloma acuminata(CA) patients, including 58 cases for HPV DNA sequencing.
Results:The HPV infection rate in 316 patients was 81.6%. The first fifth genotypes of single HPV infection were HPV11, 6, 18, 16 and 33. HPV6+ 11, HPV6+ 11+ 16 and HPV6+ 18 were more common in mixed infection. The highest HPV infection rate was from the males aged below 35 years. Regarding the sequencing result as a gold standard, the sensitivity, specificity and accuracy of PCR-RDB in HPV were 92.31%, 89.47% and 91.38% respectively.
Conclusions:The proportion of HPV crissum infection in young male was quite high, the major pathogenic types were HPV 11, 6, 18, 16 and 33. The PCR-RDB method can meet the needs of clinical diagnosis of HPV.
