Analysis of Lamivudine-resistant variants mutation patterns in patients with chronic hepatitis disease
10.3760/cma.j.issn.1003-9279.2017.01.006
- VernacularTitle: 慢性肝病患者拉米夫定耐药毒株突变模式特征分析
- Author:
Yabo OUYANG
1
;
Qing LI
;
Feili WEI
;
Yulin ZHANG
;
Yanxiang HUANG
;
Chenzhao SONG
;
Xianghua GUO
;
Fang XIE
;
Shanshan WANG
;
Dexi CHEN
Author Information
1. Beijing Institute of Hepatology, You An Hospital Affiliated to Capital Medical University, Beijing 100069, China
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Chronic hepatitis diseases;
Lamivudine;
Drug-resistant mutation;
S gene mutants
- From:
Chinese Journal of Experimental and Clinical Virology
2017;31(1):31-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the genetic characteristics of Lamivudine-resistant mutation patterns and HBV S gene mutants in patients with chronic hepatitis disease of different disease progression.
Methods:Blood samples of LAM-resistant patients with chronic hepatitis disease were collected. HBV RT gene nucleotide sequences were obtained, and then differences in drug-resistant mutation patterns, drug susceptibility and HBV S gene mutants characteristics between the two groups were analyzed.
Results:Forty-seven chronic hepatitis B (CHB) patients and 16 HBV-related liver cirrhosis (LC)/HBV-related hepatocellular carcinoma (HCC) patients were included in this study. M204I single point mutation and L180M+ M204I/V were the most common pattern during patients with chronic hepatitis disease (35/63, 55.56%). The numbers of resistant to three nucleos(t)ide analogues in LC/HCC group was higher than CHB group’s (62.50% vs 34.04%, P=0.046). In HBV S gene, more immune associated HBsAg-escape mutations were detected in LC/HCC group than that in CHB group (62.50% vs 31.91%, P=0.031). I126T/V and G145A (for LCC/HCC group, 60%), I126S/T and S117T (for CHB group, 46.67%) were showed as the most common form for HBsAg escape mutations in the two groups. The two groups both detected RT mutations concomitantly with stop codon mutations in S gene (rtA181T/sW172* and rtM204I/sW196*).
Conclusions:Different characteristics in Lamivudine-resistant mutations and associated HBV S gene mutants were found in patients with chronic hepatitis disease of different disease progression, and LC/HCC patients exhibit more multi-drug resistant variants and immune associated HBsAg-escape mutants than CHB patients.
