Effect of Shouwu Jiangzhi decoction on hyperlipidemia Golden Syrian hamsters based on TNF-α/MAPK/apoB-48 pat
10.11665/j.issn.1000-5048.20190614
- VernacularTitle:基于TNF-α/MAPK/apoB-48通路研究首乌降脂汤对高脂血症金黄地鼠血脂的影响
- Author:
Bin LOU
1
;
Yunshan WANG
;
Minglin GU
;
Dawei WANG
Author Information
1. 江苏省中医药研究院转化医学实验室
- Publication Type:Journal Article
- Keywords:
Shouwu Jiangzhi decoction;
hyperlipidemia;
TNF-α;
apoB-48;
MAPK pathway
- From:
Journal of China Pharmaceutical University
2019;50(6):728-733
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the mechanism of Shouwu Jiangzhi decoction in treatment of hyperlipidemia by suppress apoB-48 in small intestines, Golden Syrian hamsters were randomly devided into blank group, model group, fenobrate treatment group and Shouwu Jiangzhi decoction treatment group based on weight. The hyperlipidemia models of golden Syrian hamsters were induced by high fat diet(HFD)treatment for 4 weeks, then administered orally with drugs for 4 weeks. The serum indexes of HDL-C, LDL-C, TG and TC were determined by microplate methods, ELISA kits were used to evaluate the contents of serum TNF-α, apoB-48 and FFA. The protein expression levels of p38, ERK, JNK, SREBP, TNF-α and apoB-48 in small intestines were determined by Western blots. The results showed that Shouwu Jiangzhi decoction can effectively increase the serum HDL-C level and reduce the serum level of TG, LDL-C, TNF-α and apoB-48 in HFD-induced hamsters. Furthermore, Shouwu Jiangzhi decoction can significantly downregulate the protein expressions of p38, JNK, ERK, SREBP, TNF-α and apoB-48 in small intestines. Results above indicate that Shouwu Jiangzhi decoction may downregulate the protein expression of apoB-48 to treat hyperlipidemia via partially downregulating TNF-α/MAPK signal pathway.
