Gypenoside granules improved lipid metabolism in C57BL/6J mice with hyperlipidemia
10.11665/j.issn.1000-5048.20190612
- VernacularTitle:绞股蓝总苷颗粒改善高脂血症C57BL/6J小鼠脂代谢的研究
- Author:
Yaodan ZHANG
1
;
Xinyu JIANG
;
Lanjie CAO
;
Jie WANG
;
Cuihua JIANG
;
Mengge ZHAO
;
Jian ZHANG
;
Zhiqi YIN
Author Information
1. 万邦德制药集团杭州医药技术有限公司
- Publication Type:Journal Article
- Keywords:
Gynostemma pentaphyllum;
gypenosides granules;
drug combination;
hyperlipidemia;
reverse cholesterol transport
- From:
Journal of China Pharmaceutical University
2019;50(6):713-720
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the hypolipidemic effects of gypenosides granules and its combination with lipitor, a model of hyperlipidaemia C57BL/6J mice was established by high-fat diet feeding for 4 weeks. The mice were randomly divided into blank group, model group, lipitor group(10 mg/kg of lipitor), low dose group(90 mg/kg of gypenosides granules), medium dose group(120 mg/kg of gypenosides granules), high dose group(180 mg/kg of gypenosides granules)and the combination group(180 mg/kg of gypenosides granules and 10 mg/kg of lipitor). After 4 weeks of continuous administration, the contents of serum lipid indexes, serum ALT, AST and apolipoprotein B(ApoB)were measured. The liver tissues of mice were observed by H&E staining. The expression levels of key factors involved in hepatic cholesterol metabolism were observed by RT-PCR and Western blot methods, such as adenosine triphosphate combined box transporter A1(ABCA1), liver X receptor(LXRα), cholesterol 7 alpha hydroxylase(CYP7A1)and type BΙ scavenger receptor(SR-BΙ). The results revealed that gypenosides granules significantly decreased the mice body weight, total abdominal fat area and the level of serum total cholesterol(TC). The combination group showed a more significant reduction in TC level than the other administration groups. Moreover, gypenosides granules treatment remarkably increased the protein expression of ABCA1 and up-regulated the mRNA expression of ABCA1, CYP7A1 and SR-BI. The above results suggest that gypenosides granules can significantly reduce blood lipid contents, and the combination therapy with lipitor show better the lipid-lowering effect. Meanwhile, gypenosides granules can decrease the level of serum transaminase. Preliminary exploration suggests the lipid-lowering mechanism of gypenosides granules may be involved in cholesterol reversal to regulate the level of TC.
