Design and antitumor effect of nitrated T helper cell epitope vaccine CD47-NitraTh
10.11665/j.issn.1000-5048.20190516
- VernacularTitle:硝基化通用T细胞表位疫苗CD47-NitraTh的设计与抑瘤活性
- Author:
Jianhua LUO
1
;
Xuefei XIA
;
Wenbing YAO
;
Hong TIAN
Author Information
1. 中国药科大学生命科学与技术学院 江苏省生物药物成药性研究重点实验室
- Publication Type:Journal Article
- Keywords:
immune checkpoint;
CD47;
universal T cell epitope;
tumor vaccine
- From:
Journal of China Pharmaceutical University
2019;50(5):614-621
- CountryChina
- Language:Chinese
-
Abstract:
Using the genetic code extension technology, the immunogenic amino acid, p-nitrophenylalanine, was introduced into the universal T cell epitope and then fused with the fragment of the extracellular region of the immune checkpoint molecular CD47(19-140)to construct a vaccine targeting CD47. The CD47-NitraTh vaccine elicited high titer antibody in BALB/c mice, significantly inhibited CT26 colon cancer cells growth, and increased the ratio of spleen CD4+ T cells and CD8+ T cells. Meanwhile, it promoted the polarization of naï ve T cells to Th1 cells. Notably, CD47-NitraTh not only increased the proportion of tumour-infiltrating lymphocytes but also reduced the proportion of Treg cells in tumour tissues, which means that CD47-NitraTh vaccine can remodel the tumour immunosuppressive microenvironment. The results of this study suggested that CD47-NitraTh can be used as an effective tumour vaccine candidate.
