Effect and mechanism of FGF21 on astrocyte damage induced by Aβ25-35
10.11665/j.issn.1000-5048.20190415
- VernacularTitle:GF21对Aβ25-35导致星形胶质细胞损伤的保护作用及机制研究
- Author:
Yan SUN
1
;
Xiangdong GAO
;
Song CHEN
Author Information
1. 中国药科大学生命科学与技术学院 江苏省生物药物成药性研究重点实验室
- Publication Type:Journal Article
- Keywords:
fibroblast growth factor 21;
Alzheimer′s disease;
astrocytes;
reactive oxygen species;
mitogen-activated protein kinases
- From:
Journal of China Pharmaceutical University
2019;50(4):490-496
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects and mechanism of fibroblast growth factor 21(FGF21)on astrocytes in AD-like lesions, Aβ25-35 was used to induce astrocyte model damaged. Cell model was established by inducing C6 astrocyte cell line and primary astrocyte damage with Aβ25-35. Different concentrations of FGF21 were used to intervene cell injury model induced by Aβ25-35, and cell viabilities were detected by MTT assay. Effects of FGF21 and Aβ25-35 on reactive oxygen species(ROS)levels in C6 cells were tested using DCFH-DA probe and flow cytometry. Western blot was used to assess the effects of FGF21 and Aβ25-35 on the activities of mitogen-activated protein kinases(MAPKs)in C6 cells. The results showed that FGF21 could reduce the damage of C6 cells and primary astrocytes induced by Aβ25-35, down-regulate the abnormal ROS level in C6 cells, and alleviate the abnormal phosphorylation levels of MEK1/2, ERK1/2 and p38 in C6 cells induced by Aβ25-35, suggesting that FGF21 may attenuate Aβ25-35-induced astrocyte damage by regulating ROS pathway and MAPKs signaling pathway.
