Hair regeneration in mice was promoted by 3-methyladenine through inhibiting autophagy flux
10.11665/j.issn.1000-5048.20190412
- VernacularTitle:3-甲基腺嘌呤通过抑制自噬促进小鼠毛囊再生的作用研究
- Author:
Jing LI
1
;
Fuling LUO
;
Shengwang WU
;
Jingyuan WAN
;
Hengguang ZHAO
Author Information
1. 重庆医科大学药学院
- Publication Type:Journal Article
- Keywords:
3-methyladenine;
autophagy;
hair regeneration;
rapamycin
- From:
Journal of China Pharmaceutical University
2019;50(4):468-474
- CountryChina
- Language:Chinese
-
Abstract:
To explore the role of autophagy in hair follicle cycle and whether 3-methyladenine(3-MA)could promote hair regeneration in C57BL/6 mice through inhibiting autophagy flux, hair regeneration model of C57BL/6 mice was induced on the dorsal skin by depilation, and 3-MA was intraperitoneally injected to investigate hair regrowth, meanwhile vehicle and rapamycin(RAPA)were used as the controls. Results showed that 3-MA could obviously promote hair regrowth in depilated C57BL/6 mice. Furtherly, haematoxylin-eosin staining, immunohistochemical staining, immunofluorescence and Western blot tests were used to investigate the autophagy signals and the cell proliferation. Results showed that the expression of Beclin1 and LC3B II/I ratio were significantly decreased. Expression of P62 and Ki67 were increased, as well as the CD34 and CD49f double-labeled hair follicle stem cells were obviously increased inside bulge areas in 3-MA group, while contrarily in RAPA group. These results affirmed 3-MA, an autophagy inhibitor, could promote hair regeneration in depilated C57BL/6 mice by facilitating the transformation of hair follicle from telogen to anagen. 3-MA and other analogous autophagy inhibitors probably have a potential usage in future therapy in human telogen effluvium diseases.
