Involvement of Src Family Tyrosine Kinase in Apoptosis of Human Neutrophils Induced by Protozoan Parasite Entamoeba histolytica.
10.3347/kjp.2010.48.4.285
- Author:
Seobo SIM
1
;
Jae Ran YU
;
Young Ah LEE
;
Myeong Heon SHIN
Author Information
1. Department of Environmental and Tropical Medicine, School of Medicine, Konkuk University, Chungju 380-701, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Entamoeba histolytica;
neutrophil;
apoptosis;
Src tyrosine kinase
- MeSH:
*Apoptosis;
Cells, Cultured;
Entamoeba histolytica/*immunology/*pathogenicity;
GPI-Linked Proteins/metabolism;
Genistein/metabolism;
Humans;
Neutrophils/*immunology;
Protein Kinase Inhibitors/metabolism;
Pyrimidines/metabolism;
Reactive Oxygen Species/metabolism;
Receptors, IgG/metabolism;
src-Family Kinases/antagonists & inhibitors/*metabolism
- From:The Korean Journal of Parasitology
2010;48(4):285-290
- CountryRepublic of Korea
- Language:English
-
Abstract:
Tyrosine kinases are one of the most important regulators for intracellular signal transduction related to inflammatory responses. However, there are no reports describing the effects of tyrosine kinases on neutrophil apoptosis induced by Entamoeba histolytica. In this study, isolated human neutrophils from peripheral blood were incubated with live trophozoites in the presence or absence of tyrosine kinase inhibitors. Entamoeba-induced receptor shedding of CD16 and PS externalization in neutrophils were inhibited by pre-incubation of neutrophils with the broad-spectrum tyrosine kinase inhibitor genistein or the Src family kinase inhibitor PP2. Entamoeba-induced ROS production was also inhibited by genistein or PP2. Moreover, genistein and PP2 blocked the phosphorylation of ERK and p38 MAPK in neutrophils induced by E. histolytica. These results suggest that Src tyrosine kinases may participate in the signaling event for ROS-dependent activation of MAPKs during neutrophil apoptosis induced by E. histolytica.
