Identification of the long noncoding RNA_ AK096792 in cord blood as a clinical predictor for early diagnosis of bronchopulmonary dysplasia in preterm infants

Yan Zhang; Tianping Bao; Xiaotong Song; Yunjia Hao; Zhaofang Tian; Chen Song; Yazhou Sun; Weiwei Wang; Bin Zhou; Chenghe Tang; Jiaqin Wang

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Identification of the long noncoding RNA_ AK096792 in cord blood as a clinical predictor for early diagnosis of bronchopulmonary dysplasia in preterm infants

VernacularTitle: 长链非编码RNA_AK096792作为早产儿支气管肺发育不良早期诊断的脐带血清标志物研究
Author: Yan ZHANG ¹ ; Tianping BAO ² ; Xiaotong SONG ¹ ; Yunjia HAO ¹ ; Zhaofang TIAN ² ; Chen SONG ³ ; Yazhou SUN ⁴ ; Weiwei WANG ⁴ ; Bin ZHOU ¹ ; Chenghe TANG ⁴ ; Jiaqin WANG ⁵
Author Information

1. Department of Pediatrics, Xuzhou Central Hospital(the Affiliated Xuzhou Hospital of Medical College of Southeast University), Xuzhou 221000, Jiangsu Province, China
2. Department of Neonatology, Huai′an First Hospital Affiliated to Nanjing Medical University, Huai′an 223300, Jiangsu Province, China
3. Hematology Laboratory, the First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China
4. Department of Neonatology, the First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China
5. Department of Pediatrics, the Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453000, Henan Province, China
Publication Type:Journal Article
Keywords: Infant, preterm; Bronchopulmonary dysplasia; Biomarker; Long noncoding RNA; Very low birth weight infant; Clinical predictor
From: Chinese Journal of Applied Clinical Pediatrics 2018;33(14):1075-1078
CountryChina
Language:Chinese
Abstract: Objective:To investigate the feasibility of long noncoding RNA (lncRNA)_AK096792 as a clinical predictor of bronchopulmonary dysplasia (BPD) in preterm infants.
Methods:All the cord blood(2-5 mL) of very low birth weight (VLBW) preterm infants born in Huai′an First Hospital Affiliated to Nanjing Medical University were collected from December 1, 2015 to December 1, 2017.Moreover, the peripheral blood(2 mL) of those VLBW infants diagnosed with BPD was also collected.A total of 36 infants with BPD were collected.Another 36 cases of premature children with VLBW were chosen as control group according to random number table.The relative content of lncRNA_AK096792 in cord blood and peripheral blood was detected by using real-time quantitative PCR (qPCR). Additionally, the correlation of lncRNA_AK096792 levels between cord and peripheral blood of BPD infants was analyzed.The sensitivity and specificity of lncRNA_AK096792 for BPD were analyzed by using receiver operating curve test.
Results:(1)LncRNA_AK096792 was a common, evolutionarily conserved, non-coding RNA present in both mouse and human.(2) The expression level of lncRNA_AK096792 in peripheral blood was significantly higher than that in cord blood in BPD group[(463.3±352.0)% vs.(50.0±37.5)%], and the difference was significant(P<0.001), and they were highly correlated (r=0.825, P<0.001). (3) The level of lncRNA_AK096792 in cord blood in BPD group was signi-ficantly higher than that in non-BPD group [(484.3±280.5)% vs.(101.2±28.6)%], and the difference was significant(P<0.001). (4)When lncRNA_AK096792 served as a clinical predictor for BPD, the specificity was 83.3%, the sensitivity was 75.6%, and an area under the receiver operating characteristic curve was 0.88(P<0.001).
Conclusions:LncRNA_AK096792 is highly correlated with the development of BPD.The level of lncRNA_AK096792 in umbilical cord blood of premature infants can be used as an early predictive marker for BPD, it calls for further study.