Effect of GSK-3β inhibitor on the expression of RANK-RANKL in rats kidney tissue with diabetic nephropathy
10.3760/cma.j.issn.0529-5807.2018.12.010
- VernacularTitle: GSK－3β抑制剂氯化锂对糖尿病肾病大鼠肾组织核因子κB受体活化因子与其配体表达的影响
- Author:
Yixia ZHOU
1
;
Yonghong GUO
;
Long LI
;
Lisa LYU
;
Ying QIN
;
Xiaojie LI
;
Kun XU
;
Yanni YU
Author Information
1. Department of Pathology, Affiliated Hospital, Guizhou Medical University, Guiyang 550004, China
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Lithium chloride;
Rats
- From:
Chinese Journal of Pathology
2018;47(12):945-950
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and significance of GSK-3β inhibitor(LiCl)and RANK-RANKL on the renal tissue of diabetic nephropathy(DN) rats.
Methods:SD rats were divided into normal control group (NC), DN model group (DN) and GSK-3β inhibitor intervention group (LiCl). Twenty-four hour urine protein of rats were determined by Coomassie brilliant blue. Kidney tissue sections were stained by HE. The expression of GSK-3β, RANK and RANKL protein were determined by immunohistochemistry staining. The mRNA of GSK-3β, RANK, RANKL was detected by RT-qPCR.
Results:Compared with NC group[(14.72±3.37)g], the level of 24-hour urinary protein[(154.17±20.65)g] increased significantly in DN group; compared with DN Group, the level of 24-hour urinary protein [(107.22±31.15)g]decreased in LiCl group(P<0.05). Compared with NC group(2.10±0.60, 1.10±0.20, 1.21±0.20; 19.52±3.20, 1.80±1.10, 1.81±0.50), the pathological changes of renal tissues of DN group aggravated, the mRNA and expression of protein of GSK-3β, RANK and RANKL increased(9.10±2.15, 8.95±2.40, 9.90±2.60; 32.70±7.20, 19.20±4.32, 20.92±5.90); compared with DN group, the pathological changes of renal tissues of LiCl group alleviated, mRNA and the expression of protein of factors above declined(2.70±0.80, 2.32±0.65, 3.58±1.10; 22.35±3.25, 4.20±2.42, 5.90±2.36; P<0.05).
Conclusion:RANK and RANKL play an important role in the development of DN, LiCl influence Wnt and NF-κB signal pathway down-regulating RANK and RANKL to suspend development of diabetic nephropathy.
