Relationship between expression of BRAFV600E and Ki67 protein and clinical pathologic characteristics of thyroid carcinoma
10.3760/cma.j.issn.1008-6706.2018.11.028
- VernacularTitle: BRAFV600E、Ki67蛋白表达与甲状腺癌临床病理特征的相关性分析
- Author:
Gun CHEN
1
;
Linghua CONG
1
;
Laifu FANG
1
;
Shiling ZHANG
2
Author Information
1. Department of Pathology, Yinzhou People's Hospital of Ningbo, Ningbo, Zhejiang 315040, China
2. Department of Experiment Pathology, The Center for Clinical Pathology of Ningbo, Ningbo, Zhejiang 315000, China
- Publication Type:Journal Article
- Keywords:
Thyroid neoplasms;
Protein, BRAFV600E;
Protein, Ki67
- From:
Chinese Journal of Primary Medicine and Pharmacy
2018;25(11):1467-1470
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the relationship between BRAFV600E, Ki67 protein and thyroid carcinoma with different pathologic characteristics, thus to provide clinical evidence on early prognosis and personalized treatment in patients with thyroid carcinoma.
Methods:From January 2015 to January 2017, 76 patients diagnosed as papillary thyroid carcinoma(PTC), who treated in Yinzhou People's Hospital, were enrolled.Twenty cases with normal tumor-adjacent tissue after operation and 34 patients with non-PTC were also enrolled as control.All the patients enrolled were not treated with endocrine, chemical and radiotherapy.The expression of BRAFV600E and Ki67 protein was detected by immunohistochemical method, and the BRAFV600E and Ki67 expression and their correlation with different pathologic characteristic of thyroid carcinoma was analyzed by SPSS 18.0.
Results:The positive rate of BRAFV600E and Ki67 protein in 110 patients with thyroid carcinoma was 56.36%(62 cases) and 39.09%(43 cases), with the highest expression of BRAFV600E and the lowest expression of Ki67 in patients with PTC respectively.The expression of BRAFV600E and Ki67 protein was all negative in normal tumor-adjacent tissue.The positive expression of BRAFV600E and Ki67 in patients with PTC demonstrated significant difference in TNM stage, tumor side and lymphoid node metastasis(BRAFV600E: χ2=5.281, 9.771, 9.771, all P<0.05; Ki67: χ2 =7.098, 4.070, 5.067, all P<0.05), while with no obvious difference in sex, age and numbers of tumor(BRAFV600E: χ2=0.078, 0.093, 0.061, all P>0.05; Ki67: χ2=0.224, 0.518, 1.281, all P>0.05).
Conclusion:The detection of BRAFV600E and Ki67 is benefit on judging of innocent and malignant, the malignant degree of thyroid tissue.The high expression of BRAFV600E and Ki67 is benefit on differentiating PTC and early evaluation of prognosis.
