Screening of microRNAs targeting Notch signaling pathway implicated in inner ear development and the role of microRNA-384-5p
10.3760/cma.j.issn.1673-0860.2018.11.007
- VernacularTitle: 靶向Notch信号通路与内耳发育相关microRNA的筛选及microRNA－384－5p的实验分析
- Author:
Zhibin CHEN
1
;
Maomao PU
2
;
Jun YAO
2
;
Xin CAO
2
;
Lei CHENG
1
Author Information
1. Department of Otorhinolaryngology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
2. Department of Biotechnology, School of Basic Medical Science, Nanjing Medical University, Nanjing 211166, China
- Publication Type:Journal Article
- Keywords:
Receptors, Notch;
Ear, inner;
Hair cells, auditory;
miR-384-5p
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2018;53(11):830-837
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen the key microRNAs targeting Notch signaling pathway in inner ear and investigate its potential regulating function.
Methods:The interaction network and the Core-Notch network, involved with key genes in Notch signal pathway and differential-expressed microRNAs in inner ear, were constructed by bioinformatics methods. The important microRNAs in regulating Notch signaling pathway were screened via topological and GO analysis, followed by in vivo and in vitro investigation.
Results:MiRNA-384-5p was identified as a key regulator specifically expressed in mouse brain and inner ear, which could down-regulate Notch1. The Notch1 expression was found significantly down-regulated in miRNA-384-5p-mimic-transfected HeLa cells. The dual-luciferase reporter gene assay further confirmed the effect of miRNA-384-5p on the down-regulation of Notch1 and Dll4 in Notch signaling pathway.
Conclusions:The Core-Notch network is constructed to screen microRNAs implicated in inner ear development, and miRNA-384-5p is screened and verified to be target-regulating the Notch signaling pathway, which could be the potential target in the regeneration of impaired hair cells.
