Effect of miR-194-3p on migration of keloid fibroblasts
10.3760/cma.j.issn.1009-4598.2018.11.018
- VernacularTitle: miR-194-3p对瘢痕疙瘩成纤维细胞迁移的作用
- Author:
Zhishan XU
1
;
Qiang HUI
;
Wei LI
;
Shudan MA
;
Bingyu GUO
;
Peng CHANG
;
Kai TAO
Author Information
1. Department of Plastic Surgery, the General Hospital of Shenyang Military Region, Shenyang 110016, China
- Publication Type:Journal Article
- Keywords:
Keloid;
Fibroblast;
Migration;
miR-194-3p;
RUNX2
- From:
Chinese Journal of Plastic Surgery
2018;34(11):964-970
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of miR-194-3p on the migration of keloid fibroblasts.
Methods:Differentially expressed miRNA were screened by gene chip in 8 human keloid and normal tissues. The down regulated miR-194-3p was selected for study and its binding to RUNX2 was predicted by MiRDB, and verified by fluorescent reporter gene in human keloid fibroblasts (HKFs) and passage 3 keloid cells, respectively. The effect of miR-194-3p on the migration of fibroblasts was detected by transwell assay. Western blot and real-time PCR were used to analyze the effect of miR-194-3p on RUNX2 and MMP2 expression in HKFs. The results were analyzed by SPSS 19.0 software and compared by non-paired t test. P<0.05 was considered statistically significant.
Results:There were abnormal expressions of miR-721, miR-21-3p, miR-382-3p, miR-194-3p, miR-3107-5p and miR-144-5p in keloid. miRDB software analysis showed that miR-194-3p and RUNX2 had targeted binding sites. Reporter gene experiments showed that miR-194-3p inhibited the activity of RUNX2 by about 50% compared with the control group (t=2.7764, P=0.0005). MiR-194-3p could significantly inhibit the expression of RUNX2 and MMP2, and inhibited the migration of keloid fibroblasts (t=2.7764, P<0.05).
Conclusion:MiR-194-3p may inhibit the migration of fibroblasts by inhibiting the activity of RUNX2 in keloid.
