Role of N6-methyladenine DNA modification in the pathogenesis of infantile hemangioma
10.3760/cma.j.issn.1009-4598.2018.11.017
- VernacularTitle: DNA腺嘌呤N6甲基化修饰在婴幼儿血管瘤发病机制中的作用
- Author:
Linfeng ZHANG
1
;
Jian ZHANG
;
Renrong LYU
;
Xiaowen LIU
;
Yiliang WU
;
Guangqi XU
;
Jianhai BI
;
Ran HUO
Author Information
1. Department of Burn and Plastic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China
- Publication Type:Journal Article
- Keywords:
N6-methyladenine;
DNA methylation;
Infantile hemangioma;
Pathogenesis
- From:
Chinese Journal of Plastic Surgery
2018;34(11):959-964
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether N6-methyladenine DNA(6-mA DNA) modification is related to the occurrence of infantile hemangiomas (IH) at the epigenetic level.
Methods:The genomic 6-mA DNA data were obtained by MeDIP and high-throughput sequencing. The 6-mA DNA methylation levels in 3 proliferative hemangioma specimens and adjacent skin tissues were compared by u-test. The functional differences of 6-mA modified genes were analyzed by GO analysis.
Results:The level of 6-mA DNA modification in IH tissue was higher. The coverage of 6-mA Peaks in the genome was 0.037% in the tumor tissue, and the coverage of 6-mA Peaks in the surrounding skin tissue was 0.013% in the genome. The difference between the two groups was statistically significant (u=5999.87, P=0.00). The gene functions of differentially 6-mA modified genes in tumors were enriched in mesoderm development, stem cell differentiation, mesenchymal development, and cell cycle. These gene functions were closely related to the pathogenesis of IH.
Conclusion:Abnormal 6-mA DNA modification may be one of the pathogenesis of infantile hemangioma.
