Molecular detection and genotyping of human papillomavirus
10.3760/cma.j.issn.0253-3766.2018.10.003
- VernacularTitle: 人乳头状瘤病毒核酸分型检测试剂的研究进展
- Author:
Yabin TIAN
1
;
Chuntao ZHANG
1
Author Information
1. DivisionⅡ of In Vitro Diagnostics for Infectious Diseases, National Institutes for Food and Drug Control, Beijing 100050, China
- Publication Type:Review
- Keywords:
Human papillomavirus;
Genotyping;
Cytology;
Target amplification
- From:
Chinese Journal of Oncology
2018;40(10):729-735
- CountryChina
- Language:Chinese
-
Abstract:
The development of cervical cancer is associated with persistent infection of high-risk human papillomavirus. The detection and genotyping of HPV could be used to evaluate the epidemiology of HPV infections, monitor HPV vaccine efficacy, and screen cervical cancer. There are a lot of commercially available molecular tests for HPV, based on different methodologies and detection systems. In principle, it mainly includes two categories, namely signal amplification and target amplification. Most of them are based on PCR amplification, such as fluorescent PCR, PCR-reverse hybridization, etc. The performances of detection reagents are different. In addition, HPV genotyping assays based on next-generation sequencing and quantitative HPV detection kits are developed. However, only a small number of commercial assays have been clinically verified. A large number of assays which may bring greater values in the screening of cervical cancer are needed to be clinically validated.
