Determination of p-Phenylene diamine in workplace air by elution solution-liquid chromatography
10.3760/cma.j.issn.1001-9391.2018.09.020
- VernacularTitle: 工作场所空气中对苯二胺的溶液洗脱-液相色谱测定法
- Author:
Mengmeng LIU
1
;
Baofeng LIU
;
Jian ZHANG
;
Jing LIU
;
Ming ZHANG
;
Shulan ZHAO
Author Information
1. Institute of occupational Disease control and Prevention, Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China
- Publication Type:Journal Article
- Keywords:
Air Of Workplace;
p-Phenylene Diamine (PPD);
High Performance Liquid Chromatography
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(9):700-703
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a elution solution-liquid chromatography method for determination of p-Phenylene diamine (PPD) in workplace air.
Methods:p-Phenylene diamine (PPD) in the air of workplace was collected with glass fiber filters coated with dilute sulfuric acid and extracted with an aqueous EDTA solution. The target toxicant was separated with the C18 column and analyzed with UV detector, identified by retention time, and quantified by peak area.
Results:The linear range of PPD in the air of workplace was 2.00~10.00 μg/ml, with a correlation coefficient of 0.999 96. The limit of detection was 0.07 μg/ml. The lower limit of quantification was 0.23 μg/ml. The minimum detectable concentration was 0.003 1 mg/m3 under 45.0 L sampling volume and 2.0 ml extraction solution volume. The within-run precision of different PPD concentrations was 0.15%~2.3% and the between-run precisions was 1.4%~2.6%; The extraction efficiencies was 91.4%~95.4%; The average collection efficiencies was 96.6%; The samples could be stored for 7 days isolation of air. The potential coexistence of m-Phenylene diamine and o-Phenylene diamine with p-Phenylene diamine (PPD) in the air did not interfere with the results of determination.
Conclusion:This method has high sensitivity, precision, accuracy and lower limit of detection and it is applicable for determination of p-Phenylene diamine (PPD) in workplace air.
