Effects of oxymatrine on oxidative stress and inflammatory reaction after cerebral ischemia-reperfusion in rat
10.3760/cma.j.issn.1673-4246.2018.08.011
- VernacularTitle: 苦参素对脑缺血再灌注模型大鼠氧化应激和炎症反应的影响
- Author:
Yafei HAN
1
;
Ting YU
Author Information
1. Fourth Department of Neurosurgery, Handan Central Hospital, Handan 056001, China
- Publication Type:Journal Article
- Keywords:
Matrine;
Brain ischemia;
Reperfusion injury;
Oxidative stress;
Inflammatory;
Rats
- From:
International Journal of Traditional Chinese Medicine
2018;40(8):727-732
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of oxymatrine on oxidative stress and inflammatory reaction after cerebral ischemia reperfusion in rat.
Methods:A total of 140 SD rats were randomly divided into the sham group, model group and oxymatrine low, medium, high-dose groups (28 in each group). Beyond the sham group, the other groups were established the rat model of focal cerebral ischemic reperfusion with Zea-Longa occluded suture. The the low-, medium- and high-dose matrine groups were given 25, 50, and 100 mg/kg oxymatrine solutions injected intraperitoneally from the second day after operation for 7d. , The model group and the sham group were injected with an equal volume of saline. The neurological deficits, ratio of infarct volume, water content of the brain were evaluated; the morphological changes of brain tissue were observed by HE Staining, and the cell apoptosis were observed after TUNEL staining; the activity of antioxidant enzymes and malondialdehyde (MDA) in brain tissue were measured by biochemical analysis; the content of NO and the activity of iNOS were measured; the expression of NF-ĸB were determined by Western blotting. The inflammatory cytokines in brain tissue were determinated.
Results:Compared with the model group, the neurological function score, brain tissue water content, infarct volume and apoptotic index decreased in the rats of oxymatrine medium-, high-dose groups (P<0.05 or P<0.01). The activity of SOD (148.68 ± 9.12 U/mg, 161.34 ± 10.09 U/mg vs. 140.63 ± 8.47 U/mg), CAT (3.90 ± 1.32 U/mg, 4.15 ± 1.47 U/mg vs. 2.73 ± 0.89 U/mg), GSH-Px (11.46 ± 2.65 U/mg, 13.59 ± 3.27 U/mg vs. 9.35 ± 2.03 U/mg) were significantly increased (P<0.05 or P<0.01). The content of MDA (20.18 ± 3.59 μmol/g, 17.46 ± 3.21 μmol/g vs. 29.86 ± 5.40 μmol/g), iNOS (12.64 ± 1.83 U/mg, 11.75 ± 1.62 U/mg vs. 14.17 ± 2.06 U/mg), NO (23.64 ± 2.18 μmol/g, 21.27 ± 2.03 μmol/g vs. 27.82 ± 2.42 μmol/g), TNF-α (43.9 ± 6.3 nmol/L, 37.2 ± 5.8 nmol/L vs. 56.3 ± 6.9 nmol/L), IL-1β (715.0 ± 68.5 nmol/L, 683.9 ± 70.8 nmol/L vs. 930.8 ± 91.4 nmol/L), IL-6 (168.4 ± 22.5 nmol/L, 133.7 ± 18.1 nmol/L vs. 212.5 ± 24.7 nmol/L) were significantly decreased (P<0.05 or P<0.01). The expression of NF-ĸB in brain (0.35 ± 0.13, 0.24 ± 0.08 vs. 0.62 ± 0.15) were significantly down-regulated (P<0.05 or P<0.01).
Conclusions:The Oxymatrine inhibits oxidative stress injury and inflammatory response after cerebral ischemia-reperfusion in rats.
