Method selection for pretreatment in direct identification of pathogen in positive blood culture by MALDI-TOF MS
10.3760/cma.j.issn.1009-9158.2018.08.002
- VernacularTitle: MALDI－TOF MS直接鉴定阳性血培养病原菌的前处理方法选择
- Author:
Hongwei PAN
1
;
Enhua SUN
Author Information
1. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China
- Publication Type:Journal Article
- Keywords:
Blood culture;
Microbiological techniques;
Spectrometry, mass, matrix-assisted laser desorption-ionization
- From:
Chinese Journal of Laboratory Medicine
2018;41(8):563-566
- CountryChina
- Language:Chinese
-
Abstract:
Bloodstream infection is one of the leading causes of death worldwide. Rapid determination of the primary pathogen is crucial for diagnosis and antibiotic therapy of bacteremia. The application of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) was a major breakthrough in microbiology. This technology is simple, rapid, economical and accurate, which has been introduced into the microbiology laboratory formicroorganism identificationwidely. Recently, MALDI-TOF MS was combined with extraction methods for direct pathogens identificationfrom positive blood cultures. The methods of pathogensextraction from positive blood cultures are crucial for the accuracy of identification. And developing a standard extraction protocolhas become a hot spot of research in recent years. (Chin J Lab Med, 2018, 41: 563-566)
