Expression of PD-L1 and Gal-9 in human adipose-derived stem cells and its influencing factors
10.3760/cma.j.issn.1009-4598.2018.08.017
- VernacularTitle: 人脂肪干细胞PD-L1和Gal-9的表达及其影响因素
- Author:
Kaijian ZHOU
1
;
Shu GUO
;
Fei LI
;
Ying QIAO
;
Guoxin LIANG
;
Xiaowei ZHANG
Author Information
1. Department of Plastic Surgery, First Hospital of China Medical University, Shenyang 110001, China
- Publication Type:Journal Article
- Keywords:
Adipose-derived stem cells;
Programmed cell death ligand 1;
Galectin 9;
Interferon-γ
- From:
Chinese Journal of Plastic Surgery
2018;34(8):661-668
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether human adipose-derived stem cells (ADSCs) express PD-L1 and Gal-9 and its potential influence factors.
Methods:ADSCs isolated from 28 healthy female donors who underwent liposuction of the abdomen or breast tissue were cultured and characterized. The expression of PD-L1 and Gal-9 were detected using flow cytometry. The impact of donor age, body mass index (BMI), donor site and interferon-γ (IFN-γ) on the expression of PD-L1 and Gal-9 was analyzed using multivariate linear regression analysis.
Results:The cultured cells fulfilled the criteria for defining MSCs according to the international standards and expressed PD-L1 and Gal-9. Breast-derived ADSCs had higher expression levels of PD-L1 (37.24%±8.20%) and Gal-9 ( 4.41%±2.65%) than those in abdomen-derived ADSCs (28.80%±8.59% and 2.51%±1.39%, respectively) (P=0.018, P=0.039). Multivariate linear regression analysis showed that donor age and site were independent risk factors affecting PD-L1 expression (P=0.009, P=0.006). The expression of PD-L1 decreased by 0.385% for every one year of age increase, and it was 8.58% higher in the breast-derived ADSCs than in the abdomen-derived ADSCs. Donor site was an independent risk factor for Gal-9 expression (P=0.041). Gal-9 positive expression rate in the breast-derived ADSCs was 1.898% higher than that in the abdomen-derived ADSCs. BMI was not a risk factor affecting PD-L1 or Gal-9 expression on ADSCs. The expression of PD-L1 and Gal-9 on ADSCs were significantly upregulated after 48 hours stimulation with 20 ng/ml IFN-γ in vitro.
Conclusions:Human ADSCs expressed PD-L1 and Gal-9. Donor age, site and IFN-γ treatment were independent risk factors affecting the expression of PD-L1 and Gal-9 on ADSCs.
