The effects of methylprednisolone on NLRP3 inflammasome in rats with acute lung injury Induced by Phosgene
10.3760/cma.j.issn.1001-9391.2018.08.003
- VernacularTitle: 甲基强的松龙对光气吸入性肺损伤大鼠NLRP3炎症小体的影响
- Author:
Fangqing ZHOU
1
;
Daikun HE
;
Yiru SHAO
;
Jie SHEN
Author Information
1. Emergency & Intensive Care Centre of Jinshan Hospital affiliated to Fudan University, Medical Research Centre for Chemical Injury, Emergency and Critical Care of Fudan University, Shanghai 201508, China
- Publication Type:Journal Article
- Keywords:
NLRP3 Inflammasome;
Phosgene;
Acute Lung Injury;
Inflammatory Response;
Methylprednisolone
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(8):573-579
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of methylprednisolone on NOD-like receptor hot protein domain-associated protein 3 (NLRP3) inflammasome in phosgene-induced acute lung injury.
Methods:Rats were randomly divided into four groups, 10 rats in Air group (inhalation of air of the same volume as the phosgene group) , 10 rats in Phosgene group (inhalation of 8.33 mg/L with 100% purity phosgene for 5 min) , 10 rats in Saline control group (inhalation of the same dose of phosgene and 2 mg/kg saline via tail vein injection one hour later) , 10 rats in MP group (inhalation of the same dose of phosgene and 2 mg/kg MP via tail vein injection one hour later) . The specimens of serum, bronchoalveolar lavage fluid (BALF) and lung tissue were collected after 6h. Morphological changes were observed by HE staining. The expression of NLRP3 in the lung of four groups was detected by immunohistochemistry. NLRP3、ASC and caspase-1 expression in the lung tissue was quantified by Western blot. Reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of NLRP3、ASC and caspase-1 mRNA in the lung tissue. The concentrations of IL-1β、IL-18 and IL-33 in the serum and BALF were measured by enzyme-linked immunosorbent assay.
Results:We successfully replicated the model of phosgene-induced ALI in rats. Morphological of HE staining after phosgene exposure to 6 h observed inflammatory cell infiltration in lung tissue in Phosgene group. Immunohistochemical staining results showed that there were many NLRP3 positive cells in lung tissue in Phosgene group. The levels of NLRP3, caspase-1 mRNA and protein expression in lung were significantly increased (P<0.05) in Phosgene group compared with Air group; compared with Phosgene group, The levels of NLRP3 and caspase-1 mRNA and protein expression in MP group were significantly decreased (P<0.05) . Compared with Air group, The levels IL-1β、IL-18 and IL-33 mRNA protein expression in the serum and BALF were significantly increased (P<0.05) in Phosgene group. Compared with Phosgene group, The levels IL-1β、IL-18 and IL-33 mRNA protein expression in the serum and BALF were significantly decreased (P<0.05) in MP group.
Conclusion:Methylprednisolone can effectively protect the rats from phosgene-induced acute lung injury by inhibiting the expression of the NLRP3 inflammasome and reducing the release of inflammatory factors such as interleukin-1β (IL-1β) mediated by it.
