Identification of taenia spp adults and cysticercose cysts in human by using multiplex PCR
- Author:
Vien Viet Ha
;
Dao Due Le
;
Manh Due Nguyen
;
Tan Van Hoang
;
Nguyen Hanh Doan
;
Nhung Thi Vu
- Publication Type:Journal Article
- Keywords:
Taenia spp adults;
cysticercose cysts;
multiplex PCR
- MeSH:
Taenia spp adults;
cysticercose cysts;
multiplex PCR
- From:Journal of Malaria and parasite diseases Control
2003;0(1):62-69
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: The multiplex polymerase chain reaction can rapidly differentiate taenia spp adults and cysticercose cysts.\r\n', u'Objective: The study aimed to identify taenia spp adults and cysticercose cysts in human by using multiplex PCR\r\n', u'Subjects and methods: The multiplex PCR was applied by using the 4 forward and one reverse primer to amplify the target gene cytochrome c oxydase subunit I (COXI) of the Taenia spp in human. T\r\n', u'Results:4 molecular sizes of PCR products were appeared: 269 bp,720 bp, 827 bp, 984 bp. Seventy six samples including 65 flat worms and 11 cysticercose cysts which collected from the patients who are living in 19 different provinces and cities of Northern part of Viet Nam and treated in the clinic of NIMPE were examined. Of 65 flat worms analyzed 35 samples were T.asiatica (58.46%), 27 were T.saginata (41.54%). All 11 cysticercose cysts were T.solium (100%). \r\n', u'Conclusion: The result also indicated that some time 3 Taenia species were found at the same area. A remarkable difference of infection rate was found between men and women, also adults and children. \r\n', u'\r\n', u'
