Effect of CKIP-1 siRNA lentivirus transfection on proliferation of glioma U87-MG cells
10.3760/cma.j.issn.1674-6554.2018.07.003
- VernacularTitle: 酪蛋白激酶2相互作用蛋白1基因沉默对胶质瘤U87-MG细胞增殖的影响
- Author:
Shixiang CHENG
1
;
Qian ZHANG
2
;
Tailong YI
1
;
Lei ZHU
1
;
Haoxiang XU
1
;
Yanguo XI
3
;
Wenbin ZHANG
1
Author Information
1. Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery, Affiliated Hospital of the Logistics University of Chinese People’s Armed Police Force (PAP), Tianjin 300162, China
2. Department of Anesthesiology, General Hospital of PAP, Beijing 100039
3. Department of Neurosurgery, Cang Zhou Central Hospital, Cangzhou 061001, China
- Publication Type:Journal Article
- Keywords:
Casein kinase 2-interacting protein 1;
Glioma;
Gene silencing;
Cell proliferation
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2018;27(7):588-592
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Casein kinase 2-interacting protein 1 (CKIP-1) gene silencing on the proliferation of glioma cells U87-MG.
Methods:The recombinant lentiviral vectors targeting CKIP-1 gene or negative control were constructed and then used to infect glioma U87-MG cell line.The effects of knock-down on the mRNA or protein expression of CKIP-1 were evaluated by real-time qPCR and western blotting.Cell cycle was detected by the flow cytometry assay, and cell proliferation changes were evaluated by cell counting, MTT, and BrdU assay, respectively.Lastly, the colony formation was used to investigate the effect of CKIP-1 knock-down on the clone formation.
Results:Compared with the group of Ctrl, CKIP-1 siRNA was observed to significantly inhibit CKIP-1 expression at the mRNA levels (Ctrl (1.01±0.13) vs CKIP-1 siRNA (0.23±0.02), P<0.01) and protein levels in the U87-MG cells.Also, CKIP-1 suppression mediated by RNAi decreased the ratio of G0/G1 phase (Ctrl (69.64±0.79) vs CKIP-1 siRNA(62.64±0.66), P<0.01), increased that of G2/M phase (Ctrl (8.36±0.52) vs CKIP-1 siRNA(13.87±2.90), P<0.05), and significantly inhibited the cell proliferation and clone formation (Colony number: Ctrl (25±2) vs CKIP-1 siRNA(2±1), P<0.05) of transfected U87-MG cells.
Conclusion:Knocking down the expression of CKIP-1 significantly inhibit cell proliferation in human U87-MG glioma cells, indicating that CKIP-1 is involved in the development of gliomas and could promote the cell proliferation.
