The role of liquid biopsy of cerebrospinal fluid in the diagnosis and treatment of central nervous system tumors
10.3760/cma.j.issn.1009-9158.2018.07.014
- VernacularTitle: 基于脑脊液的液体活检在中枢神经系统肿瘤诊治中的作用
- Author:
Xiu HUANG
1
;
Ming GUAN
Author Information
1. Medicine Laboratory, Shanghai Fudan University Hua Shan Hospital, Shanghai 200040, China
- Publication Type:Review
- Keywords:
Central nervous system neoplasms;
Liquid Biopsy;
Cerebrospinal Fluid;
Neoplastic cells, circulating
- From:
Chinese Journal of Laboratory Medicine
2018;41(7):554-558
- CountryChina
- Language:Chinese
-
Abstract:
The central nervous system tumor is a common tumor seen both in children and adult. It is difficult to remove by surgery, and its mortality rate is high. Early diagnosis and real-time monitoring of this tumor are difficult due to drawbacks of the current laboratory tests (imaging, cell morphology, cytogenetics), such as low sensitivity, low positive rate and difficulty in sampling. The cerebrospinal fluid, which can circulate rapidly between ventricle and spinal cord pool, is fully in contact with the central nervous system, thus containing a great number of metabolic materials from the central nervous system. For this reason, the cerebrospinal fluid is an ideal sample for detecting central nervous system tumors. Compared with the conventional biopsy, the liquid biopsy of cerebrospinal fluid has many advantages, such as less-invasive, sampling successively, reflecting the progression of disease immediately and adjusting the treatment regimen flexibly. This paper will briefly review the molecular targets of liquid biopsy such as DNA, RNA and circulating tumor cells, elaborate the role of novel technologies including next-generation sequencing and digital PCR in the diagnosis and treatment of the central nervous system tumors.(Chin J Lab Med, 2018, 41: 554-558)
