The establishment of a chyle testing method based on n-hexane extraction system
10.3760/cma.j.issn.1009-9158.2018.07.009
- VernacularTitle: 一种基于正己烷萃取系统的乳糜实验方法的建立
- Author:
Beihan WANG
1
;
Ruiling WANG
;
Xiaoli ZHANG
;
Cui WANG
;
Qi DING
;
Liping LIU
;
Rong ZHAO
;
Xiaoli YANG
Author Information
1. Clinical Laboratory, the General Hospital of People′s Armed Police Forces, Beijing 100039, China
- Publication Type:Journal Article
- Keywords:
Chyle;
Hexanes;
Ethyl ethers;
Clinical laboratory techniques
- From:
Chinese Journal of Laboratory Medicine
2018;41(7):527-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To set up a chyle (n-hexane) testing method based on n-hexane extraction system.
Methods:The hydrothorax specimen which was positive in chyle testing was selected and the feasibility was evaluated by replace Diethyl ether with n-hexane. The optimal extraction volume ratio for the new chyle testing was confirmed and sultan Ⅲ dye process was improved. Then, the new chyle testing method and its standard operation process was established.A total of 120 specimens with triglyceride(TG)in different levels were collected and divided into three groups with 40 specimens for each group (group A. TG≤1.7 mmol/L; B. 5.6
Results:N-hexane can replace Diethyl ether in the Chyle test. The extraction volume ratio of 1 ml sample plus 400 μl N-hexane is the best one and the sultan Ⅲ tube dying method is better than former direct smear. Compared with the old Chyle test using Diethyl ether, the new Chyle test using N-hexane is in good consistency with the Kappa value 0.95. The clinical coincidence rate of the above Chyle test reaches 94.8%(127/134) after the verification of 134 specimens.
Conclusions:A new N-hexane test is established successfully. It was convenient to operate with high security and little contamination and thus has a wide clinical application value.(Chin J Lab Med, 2018, 41: 527-530)
