Effect of Mercury Chloride on Humoral and Cell-mediated Immune Responses in Mice.
- Author:
Jung Ho YOUM
- Publication Type:In Vitro ; Original Article
- Keywords:
mercury chloride;
immune response
- MeSH:
Animals;
Concanavalin A;
Hemagglutinins;
Immunoglobulin E;
Immunoglobulin G;
Lymphocytes;
Mice*;
Phytolacca americana;
Sheep
- From:Korean Journal of Preventive Medicine
1995;28(1):27-42
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The in vivo and in vitro humoral and cell-mediated immune responses of lymphocytes of BALB/c mouse exposed to mercury chloride(HgC12) were investigated. In vitro exposure of the splenocytes to mercury chloride produced overt cytotoxicity in 3 hours period. The IC50(the concentration required to inhibit a splenocyte viability by 50%) for mercury chloride was >0.1mM for cytotoxicity. In vivo mercury chloride exposed mice were significantly depressed delayed type hypersensitivity(DTH) response to sheep red blood cells(SRBC) in a dose-dependent manner compared with control group. Mercury chloride inhibited the proliferative responses of splenocytes to lipopolysaccharide. pokeweed mitogen, concanavalin A and phytohemagglutinin in a dose-dependent manner. Hemagglutinin response to SRBC in mercury chloride exposed mice was significantly depressed in a dose-dependent manner compared with control group. After 7 weeks of mercury chloride exposure in vivo. mercury chloride induced an increase of nonspecific serum IgG1 and IgE levels in BALB/c mice.
