Effect of Growth Hormone on Death of Sertoli Cell.
- Author:
Min Ja CHUNG
;
Jae Hong YU
- Publication Type:Original Article
- Keywords:
Sertoli cell death;
Growth hormone
- MeSH:
Animals;
Cell Culture Techniques;
Cell Death;
Growth Hormone*;
Humans;
Incubators;
Insulin-Like Growth Factor I;
Male;
Mice;
Sertoli Cells
- From:Journal of Korean Society of Pediatric Endocrinology
2002;7(2):184-189
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Growth hormone(GH) receptors have been detected in Sertoli cells and clinical observations suggested the involvement of GH action(either indirectly via IGF-I or directly) on testicular function. This study is aimed at assessing the effect of GH on cell death during Sertoli cell culture. METHODS: Sertoli cells(TM4, mouse, male, ATCC, USA) were cultured by using DMEM(Dulbecco' Modified Eagle' Medium, Biowhitteker, USA) and Fetal Bovine Serum(10%, GIBCO BRL, USA) with or without human recombinant growth hormone(Eutropin, LGCI, Korea) of different concentrations(10, 50, 100, 500 microgram/mL of media), in 34degress C, 5% CO2 incubator. After cultivation during 5 days, Sertoli cells were examined under light microscope(x400). RESULTS: Number of survival Sertoli cell was remarkably increased in the GH treated(50 microgram/mL in media) group(62.6+/-12.4) compare to control(22.4+/-4.8)(P<0.05). Degree of survival Sertoli cell was increased proportionaly until 50 microgram/mL in concentration of GH in media, but decreased in higher than it. CONCLUSION: GH has the protective effect on death of Sertoli cell and it depends on GH concentration.
