DNA-PKcs functions in cellular hyper-radiosensitivity
10.3760/cma.j.issn.0254-5098.2018.07.002
- VernacularTitle: DNA-PKcs在细胞辐射超敏感性中的作用研究
- Author:
Ji MA
1
;
Caiyong YE
2
;
Nan DING
2
;
Jiayun ZHU
2
;
Wentao HU
1
Author Information
1. School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China
2. Department of Space Radiobiology, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
- Publication Type:Journal Article
- Keywords:
Tumor cells;
Low dose hyper-radiosensitivity;
Chk2 kinase;
DNA-PKcs
- From:
Chinese Journal of Radiological Medicine and Protection
2018;38(7):489-493
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the functions of DNA-PKcs in cellular low dose hyper-radiosensitivity.
Methods:Colony-formation assay was used to detect the survival fractions of M059K and M059J cell lines treated by X-ray irradiation. Micronucleus assay and γ-H2AX foci assay were used to measure the radiation-induced DNA damage. Western blot was used to detect the relative expression levels of phospho-Chk1, total Chk1, phospho-Chk2 and total Chk2 of M059K and M059J cells.
Results:The hyper-radiosensitivity was observed in M059K cells irradiated with X-ray of doses lower than 1 Gy. DNA damage levels did not show HRS/IRR in the cell lines we used. pChk1/Chk1 in M059K cells was significantly increased during 20 min to 60 min after 0.2 Gy X-ray irradiation (t=14.157, 13.661, 14.177, 11.317, 14.512, P<0.05); pChk2/Chk2 in M059K cells was markedly increased during 20 min to 50 min after 0.2 Gy X-ray irradiation (t=13.182, 13.868, 14.155, 14.477, P<0.05).
Conclusions:M059K cells show the phenomenon of low dose hyper-radiosensitivity, which may be related to activation of proteins in G2/M phase checkpoints regulated by DNA-PKcs.
