Expression of genes associated with pattern recognition receptors in peripheral blood mononuclear cells from patients with dengue fever
10.3760/cma.j.issn.0254-5101.2018.07.009
- VernacularTitle: 登革热患者外周血单个核细胞中模式识别受体相关基因表达水平的初步研究
- Author:
Lingzhai ZHAO
1
;
Wenxin HONG
2
;
Shuang QIU
2
;
Jian WANG
2
;
Xiujie GAO
1
;
Lei YU
1
;
Fuchun ZHANG
2
Author Information
1. Institute of Infectious Diseases, Guangzhou Eighth People′s Hospital, Guangzhou 510060, China
2. Department of Infectious Diseases, Guangzhou Eighth People′s Hospital, Guangzhou 510060, China
- Publication Type:Journal Article
- Keywords:
Dengue fever;
Dengue virus;
Pattern recognition receptor;
Innate immunity
- From:
Chinese Journal of Microbiology and Immunology
2018;38(7):523-528
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the roles of pattern recognition receptors (PRRs) including Toll-like receptors (TLRs), retinoic acid-inducible geneⅠ-like receptors (RLRs) and nucleotide binding oligomerization domain-like receptors (NLRs) in the pathogenesis of dengue fever (DF) and the induction of immune responses to dengue virus.
Methods:Blood samples were collected from patients with DF at three different time points to isolate peripheral blood mononuclear cells (PBMCs) by density gradient centrifugation. Then PBMCs were used to extract ribonucleic acid (RNA). Expression of genes was detected by polymerase chain reaction (PCR) array.
Results:Expression of genes relating to PRRs signaling pathways in DF patients increased significantly in the early stage of the disease as compared with those in healthy controls, but decreased gradually during the recovery period. Expression of genes encoding TLR7 and TLR8 was enhanced at the early stage of DF. No significant changes in the expression of TLR3 and TLR9 genes were observed during the course of the disease. The genes encoding RIG-Ⅰ, MDA5 and LGP2 of RLRs family as well as NOD2 and OAS2 of NLRs family were all up-regulated.
Conclusion:Signaling pathways mediated by PRRs including TLR7/8, RIG-Ⅰ, MDA5 and so on play an important role in the pathogenesis of DF and the induction of immune responses to dengue virus.
