Influences of hypoxia inducible factor-1α on the expression of inflammatory cytokines in mice with influenza A (H1N1) virus infection
10.3760/cma.j.issn.0254-5101.2018.07.003
- VernacularTitle: H1N1病毒感染小鼠模型中HIF-1α对炎症因子表达影响的作用研究
- Author:
Peng HAN
1
;
Zhaoqin ZHU
2
;
Wanju ZHANG
2
;
Xiaoxiao MENG
1
;
Yong ZHU
1
;
Xiaohui ZHOU
2
;
Xinkun GUO
1
;
Yunwen HU
2
;
Ruilan WANG
1
Author Information
1. Department of Intensive Care Medicine, Shanghai General Hospital, Shanghai 201620, China
2. Shanghai Clinical Center of Public Health, Shanghai 201508, China
- Publication Type:Journal Article
- Keywords:
H1N1 virus;
HIF-1α
;
Inflammatory cytokines
- From:
Chinese Journal of Microbiology and Immunology
2018;38(7):494-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the changes in the expression of hypoxia inducible factor-1α (HIF-1α) and inflammatory cytokines and to investigate the role of HIF-1α in regulating the production of inflammatory cytokines during influenza A (H1N1) virus infection.
Methods:BALB/c mice were injected with H1N1 virus to establish the mouse model of H1N1 virus infection. Fifteen BALB/c mice were randomly divided into three groups: control group, H1N1 virus group and H1N1 virus+ HIF-1α inhibitor group. Inflammatory cytokines (IL-6, TNF-α, IL-1β and IL-10) in samples of serum and lung tissues were detected by Luminex and ELISA. Levels of HIF-1α in serum and lung tissue samples were detected by Western blot and ELISA, respectively.
Results:Compared with the control group, the levels of inflammatory cytokines in serum (IL-6, TNF-α, IL-1β and IL-10) and lung tissues (IL-6 and TNF-α) and the expression of HIF-1α in serum and lung tissues in the H1N1 virus group were significantly increased. The levels of HIF-1α, IL-6, TNF-α IL-1β and IL-10 in lung tissues in H1N1 virus+ HIF-1α inhibitor group were significantly lower than those of the H1N1 virus group.
Conclusion:During H1N1 virus infection, the levels of inflammatory cytokines and HIF-1α were significantly increased. The production of inflammatory cytokines was significantly reduced after inhibiting HIF-1α expression, suggesting that HIF-1α might promote the production of inflammatory cytokines.
