The effects of Xuebijing injection on apoptosis and expression of regulatory factors TNF-α、NF-κB and Caspase-3 expression in the lung tissues of acute paraquat-induced rats
10.3760/cma.j.issn.1001-9391.2018.07.021
- VernacularTitle: 血必净对急性百草枯中毒大鼠肺损伤细胞凋亡及炎性因子的影响
- Author:
Xin WANG
1
;
Aijun LI
;
Weizhe LI
;
Wenjing LU
;
Weizheng CUI
Author Information
1. Department of Emergency, the Handan Central Hospital, Handan 056001, China
- Publication Type:Journal Article
- Keywords:
Paraquat;
Respiratory distress syndrome;
Xuebijing;
Tumor neorosis factor
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(7):551-555
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of Xuebijing injection in the treatment of acute paraquat poisoning by means of studying the expression of TNF-alpha, NF-kappa B, Caspase-3 and the changes of cell apoptosis rate detected by TUNEL in the lung tissue of acute paraquat-induced rats.
Methods:On the base of random number table, 126 Wister rats weighing 220 g to 270 g were divided into 3 groups: (1) Control group: 42 rats, (2) Poisoned group: 42 rats, (3) Treatment group: 42 rats. On 1st、3rd、7th、14th、21st、28th、and 35th day, six rats from each group were anaesthetized by intraperitoneal injection of chloral hydrate. To cut the chest and take the lung tissue samples. The expression levels of Tumor Necrosis Factor-alpha, Nuclear Factor-kappa B and Caspase-3 protein in lung tissue were detected by immunohistochemical staining, as well as apoptotic cell rate was detected by TUNEL staining.
Results:The expression levels of Tumor Necrosis Factor-alpha, Nuclear Factor-kappa B, Caspase-3 protein and TUNEL staining in the lung tissue of the poisoned group was significantly higher than that of the control group (P<0.05) . Compared with the poisoned group, the expression of TNF-alpha, NF-kappa B, Caspase-3 and TUNEL in treatment group decreased significantly (P<0.05) , but they were still higher than those of the control group, and the difference was statistically significant compared with the control group (P<0.05) .
Conclusion:Apoptosis and TNF-alpha, NF-kappa B and Caspase-3 play an important role in lung injury of paraquat-induced rats. Xuebijing injection can inhibit the expression of TNF-alpha, NF-kappa B, Caspase-3 in lung tissue, reduce the apoptosis rate and alleviate the damage of lung tissue in paraquat-poisoning rats.
