Detecting the concentrations of diphenylamine in air of workplace with HPLC
10.3760/cma.j.issn.1001-9391.2018.07.019
- VernacularTitle: 工作场所空气中二苯胺测定的高效液相色谱法
- Author:
Tiantian YU
1
;
Qitao TAN
;
Yu WANG
Author Information
1. Zibo Hospital for Occupational Diseases Control and Treatment, Shandong 255000, China
- Publication Type:Journal Article
- Keywords:
Diphenylamine;
Workplace air;
High performance liquid chromatography
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(7):546-549
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the method of detecting the concentrations of diphenylamine in air of workplace with high performance liquid chromatographic (HPLC) .
Methods:According to standards of methods for determining the chemical substances in workplace air, diphenylamine in the air was collected by glass fiber filter treated with sulfuric acid, then dissolved by methanol and determined by high performance liquid chromatography with UV-detector.
Results:There was a linear relationship within the range of 0-30.0 μg/ml, and regression equation was y=8425.6x-150.7, correlation coefficient was 0.999 9, the detection limit was 0.045 μg/ml. The lowest detected concentration was 0.030 mg/m3 (sampling volume 15 L) . The within-run precision was 2.41 ℅-3.02%, the between-run precision was 3.11%-4.45%. The desorption efficiencies was 97.8℅ and the sampling efficiencies were 100%. The samples in glass fiber filter could be stored for 7 d at room temperature.
Conclusion:The present method could meet with the requirements of Guide for establishing occupational health standards-Part 4 Determination methods of air chemicals in workplace and be feasible for determination of diphenylamine in workplace air.
