Effects of the rotary cell culture system-simulated microgravity on growth features of thyroid follicular epithelial cells in rats
10.3760/cma.j.issn.1000-6699.2018.07.013
- VernacularTitle: 模拟微重力对大鼠甲状腺滤泡上皮细胞生长特性的影响
- Author:
Beining ZHANG
1
;
Jingjing LIN
;
Nan JIANG
;
Hongfeng YAN
;
Hongwei SUN
;
Bingxin XU
;
Jinlian ZHOU
;
Heming YANG
;
Yan CUI
Author Information
1. Department of General Surgery, 306 Hospital of PLA, Anhui Medical University, Beijing 100101, China
- Publication Type:Journal Article
- Keywords:
Simulated microgravity;
Thyroid follicular epithelial cells;
FRTL-5 cell line;
Proliferation;
Microfilament
- From:
Chinese Journal of Endocrinology and Metabolism
2018;34(7):598-601
- CountryChina
- Language:Chinese
-
Abstract:
The rotary cell culture system(RCCS)was used to simulate the microgravity environment, and FRTL-5 cells were divided into simulated microgravity group(SMG)and normal gravity group(NG). FRTL-5 cells were harvested after treatment for 6, 12, 24, and 36 h, the cell viability was measured by MTT assay, and the cells cycles were detected by flow cytometry. The ultrastructure of FRTL-5 cells was observed under laser confocal microscope with FITC-labeled technique. The MTT assay showed that the proliferation of FRTL-5 cells was significantly inhibited after RCCS treatment for 6, 12, 24, and 36h compared with NG(P<0.05), in which the most obvious effect was observed at 24h. The flow cytometry showed that the proportion of FRTL-5 cells at G1 stage in RCCS group was increased significantly after 6, 12, 24, and 36h compared with NG(P<0.05), while the proportion of FRTL-5 cells at S stage was decreased significantly(P<0.05)except that cultured with RCCS for 6 h. The proportion of FRTL-5 cells at G2/M stage was decreased in early phase(6-12 hours)of RCCS culture, with the lowest at 12h and transient increase at 24h of RCCS culture. The laser confocal microscope revealed that there were local microfilament depolymerization, tension fibers decrease, structure disorder, cellular pseudopodia reduction, and irregular shape among FITC-labeled FRTL-5 cells cultured with RCCS for 36h. (Chin J Endocrinol Metab, 2018, 34: 598-601)
