Effects of metformin on the expressions of ATF6 and Caspase12 in hippocampus of type 2 diabetic rats
10.3760/cma.j.issn.1000-6699.2018.07.012
- VernacularTitle: 二甲双胍对2型糖尿病大鼠海马ATF6和Caspase12表达的影响
- Author:
Yumei ZHOU
1
;
Yinmiao GUO
;
Hui FANG
;
Gang XU
;
Xiaoshuang LIU
;
Xiangbo HAO
;
Yanfeng ZHEN
Author Information
1. Graduate School North China University of Science and Technology, Tangshan 063000, China
- Publication Type:Journal Article
- Keywords:
Metformin;
Diabetes mellitus, type 2;
Activating transcription factor-6;
Caspase12
- From:
Chinese Journal of Endocrinology and Metabolism
2018;34(7):594-597
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of metformin on the expressions of activating transcription factor-6(ATF6)and Caspase12 in hippocampus of type 2 diabetic rats.
Methods:GK male rats with random blood glucose≥11.1 mmol/L were orally administrated with normal saline(DM group)and metformin(MET group, 85 mg·kg-1·d-1)for 8 weeks(n=10 each group). Wistar male rats were selected as normal contorl group(NC, n=10). After 8 weeks of continuous medication, body weight and fasting plasma glucose(FPG)were measured, and the morphology of HE stained cells and the expressions of ATF6 and Caspase12 by immunohistochemistry staining in the CA1 area of hippocampus were detected.
Results:Compared with NC group, the body weight of DM and MET groups decreased(P<0.05), but there was no significant difference between DM and MET groups(P>0.05). In comparison with NC group, FPG levels in DM and MET groups were markedly increased(P<0.05)while FPG level in MET group was significantly lower than that in DM group(P<0.05). The normal nerve cells in the CA1 region of hippocampal were lower in DM and MET groups than those in NC group, especially in DM group. The protein expressions of ATF6 and Caspase12 in DM and MET groups were higher than that in NC group(all P<0.05), and the expressions of the two protein in MET group were significantly decreased as compared with DM group(P<0.05).
Conclusion:Metformin reduces the expressions of ATF6 and Caspase12 in hippocampus of type 2 diabetic rats, which may be related to its protective effect on brain. (Chin J Endocrinol Metab, 2018, 34: 594-597)
