Clinical application value of combined detection of serum miR-378 and miR-21 in gastric cancer
10.3760/cma.j.issn.0253-3766.2018.06.008
- VernacularTitle: 血清miR-378和miR-21联合检测对胃癌的诊断价值
- Author:
Shengkai HUANG
1
;
Jia WANG
2
;
Yan LI
3
;
Hong LIN
3
;
Dongdong LI
3
;
Chanjuan CUI
1
;
Guojing WANG
1
;
Xuexiang LI
1
;
Lin YANG
4
;
Mei ZHAO
3
;
Changzhi HUANG
3
Author Information
1. Department of Clinical Laboratory, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
2. Department of Clinical Laboratory, China Meitan General Hospital, Beijing 100028, China
3. State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
4. Department of Medical Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
- Publication Type:Clinical Trail
- Keywords:
Gastric neoplasms;
miR-378;
miR-21;
Serum;
Diagnose
- From:
Chinese Journal of Oncology
2018;40(6):441-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the clinical value of combined detection of serum miR-378 and miR-21 in gastric cancer (GC).
Methods:Eighty-seven patients with GC and 78 patients with colorectal cancer(CRC) from National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences were selected, 83 individuals undergoing healthy physical examination were selected as the healthy controls. The levels of serum miR-378 and miR-21 were detected by quantitative real-time PCR (RT-qPCR) (result data were transformed as log2 for analysis).
Results:Relative expression levels of miR-378 in the serum were -1.24, -3.25 and -2.73 in healthy controls, GC and CRC patients, respectively. Compared with the healthy controls, the levels of serum miR-378 were significantly decreased in GC and CRC patients (both P<0.05). Relative expression levels of miR-21 in the serum were 0.11, 2.34 and 2.47 in healthy controls, GC and CRC patients, respectively. Compared with the healthy controls, the levels of serum miR-21 were significantly up-regulated in GC and CRC patients (both P<0.05). Moreover, the serum level of miR-378 in GC patients was inversely associated with tumor clinical stage (P<0.05). However, the level of miR-21 showed no significant differences among patients with different clinical and pathological characteristics (all P>0.05). The area under the receiver operating characteristic curve (AUC), sensitivity and specificity of miRNA-378 to diagnose GC was 0.770, 82.0% and 66.0%, respectively, and were 0.900, 85.0%, and 88.0% of miR-21, respectively. The AUC, sensitivity and specificity of combined detection of serum miR-378 and miR-21 to diagnose GC were 0.930, 92.0% and 87.0%, respectively, while the AUC of combined detection of serum CEA and CA-199 was 0.767, the AUC of combined all of the four factors was 0.946.
Conclusion:The combined detection of serum miR-378 and miR-21 have a certain effect on diagnosis of GC.
