Application and trend of development of digital PCR in virus detection
10.3760/cma.j.issn.1003-9279.2018.06.023
- VernacularTitle: 数字PCR在病毒检测中的应用及发展趋势
- Author:
Fangzhou QIU
1
;
Xinxin SHEN
2
;
Zhishan FENG
3
;
Xuejun MA
2
Author Information
1. Graduate School of Hebei Medical University, Shijiazhuang 050011, China
2. Key Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
3. Children’s Hospital of Hebei Province, Shijiazhuang 050030, China
- Publication Type:Review
- Keywords:
Digital PCR;
Virus;
Detection
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(6):664-668
- CountryChina
- Language:Chinese
-
Abstract:
In recent years, digital PCR (dPCR), as a novel nucleic acid amplification detection technique, contrasts real-time fluorescent quantitative PCR (qPCR) without the need to establish a standard curve, "single molecule template amplification" absolute quantification, insensitive to inhibitors that affect PCR efficiency, and excellent sensitivity, specificity, extremely strong repeatability and the development of the existing commercial platform technology that has great application advantages in virology and nucleic acid quantification. In this review, we address the application advantages of digital PCR in virus detection and describe its future development.
