Relationship between hepatitis C virus and serine protease inhibitor Kazal1
10.3760/cma.j.issn.1003-9279.2018.06.008
- VernacularTitle: 丙型肝炎病毒和丝氨酸蛋白酶抑制因子Kazal1型的关系研究
- Author:
Jingdi ZHOU
1
;
Guosheng GAO
1
;
Xinghui LIU
2
;
Yaoren HU
1
Author Information
1. Ningbo No. 2 Hospital, Ningbo 315010, China
2. Department of Clinical Laboratory Gongli Hospital, Shanghai 200135, China
- Publication Type:Journal Article
- Keywords:
Hepatitis C virus;
Serine protease inhibitor Kazal1;
Enzyme-linked immunosorbent assay;
Expression
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(6):599-601
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of hepatitis C virus (HCV) on the expression of serine protease inhibitor Kazal1 (SPINK1) and its clinical implication.
Methods:mRNA and protein expression of SPINK1 in Huh7.5.1 cells infected by HCV JFH-1 and the control cells were measured by RT-PCR and western blotting, SPINK1 levels in the cell supernatants and sera of HCV patients were measured by enzyme-linked immunosorbent assay (ELISA), the difference of SPINK1 levels between healthy controls and HCV patients was analyzed.
Results:Expression of SPINK1 mRNA and protein was higher in Huh7.5.1 cells infected by HCV JFH-1 than in the control cells, serum SPINK1 levels was much higher in HCV patients than in healthy controls (P=0.016).
Conclusions:HCV can upregulate the expression of SPINK1.
