Determination of the contents of two types of hemagglutinin B in quadrivalent influenza vaccines (split virion) by single radial immunodiffusion assay
10.3760/cma.j.issn.1003-9279.2018.06.005
- VernacularTitle: 单向免疫扩散测定四价流感病毒裂解疫苗中两种B型血凝素含量
- Author:
Qi SHUAI
1
;
Jun YU
;
Wenbin YANG
;
Fuqiang WU
;
Jianhua WU
;
Zhengdong JIANG
;
Xianpu YANG
;
Yue ZHAO
;
Jing ZHAO
;
Jianlin FU
Author Information
1. Jiangsu GDK Biological Technology Co., ltd, Taizhou 225300, China
- Publication Type:Journal Article
- Keywords:
Quadrivalent Influenza vaccine (split virion);
B hemagglutinin content;
Single radial immunodiffusion;
Antigen;
Linear regression
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(6):582-587
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Quadrivalent influenza vaccines contain two lineages of type B virus, this study aimed to assess whether the result of single radial immunodiffusion (SRID) are accurate. The cross-interference of two type B hemagglutinins remains unknown.
Methods:We detected the vaccine samples developed by Jiangsu GDK Biological Technology Co., ltd by SRID.
Results:There was no significant difference between the HA content of antigen reagent, bulk sample and mixed sample of two B bulk within 10 to 40 μg/ml (P>0.05). Then each hemagglutinin B was diluted respectively by other three HA, 30 μg/ml, the other hemagglutinin B or phosphate buffer solution were measured within 10-160 μg/ml. Within 10-40 μg/ml, the HA content was proportional to the diameters of immunodiffusion (R2=0.998), while within the higher content range, a ternary linear regression equation fitted best (R2=0.999).
Conclusions:No cross-interference between B/Brisbane and B/Phuket was found in SRID within both detection ranges.
