Relationship between the mutations in precore/core region of hepatitis B virus gene and the postoperative survival in hepatocellular carcinoma
10.3760/cma.j.issn.1003-9279.2018.06.003
- VernacularTitle: HBVpreC/C区基因突变与原发性肝癌预后的关系
- Author:
Chensi WU
1
;
Jianhua WU
2
;
Yue ZHAO
1
;
Yingnan WANG
1
;
Fengbin ZHANG
1
;
Weiguo ZHANG
3
;
Ruixing ZHANG
1
Author Information
1. Department of Gastroenterology, The Fourth Hospital of Hebei Medical University, Shijiazhang 050011, China
2. The Center of Laboratory Animal, The Fourth Hospital of Hebei Medical University, Shijiazhang 050011, China
3. Department of Clinical Focus, Hebei Medical University, Shijiazhuang 050011, China
- Publication Type:Journal Article
- Keywords:
HBV-HCC;
The preC/C region of HBV;
HBV-DNA;
Mutations;
Prognosis
- From:
Chinese Journal of Experimental and Clinical Virology
2018;32(6):571-575
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between the mutations in precore/core (preC/C) region of hepatitis B virus (HBV) gene and the postoperative survival in patients with hepatocellular carcinoma.
Methods:A total of 81 cases in HBV associated hepatocellular carcinoma (HBV-HCC) patients with cancer tissue genomic DNA were extracted. The preC/C region of HBV was amplified and sequenced, and survival-associated HBV mutations were identified according to the NCBI database. The relationships between the mutations in the preC/C region and HCC survival was analyzed with the Kaplan-Meier method and the Cox proportional hazards model. Eleven mutational sites were identified as statistically significant independent predictors of HBV-HCC postoperative survival.
Results:The portal vein thrombosis, tumor TNM classification and size were identified as statistically significant independent predictors of survival in HBV-HCC patients. In the research, we found that seven mutational sites in preC/C region of HBV were associated with independent risk factors for postoperative survival in patients of HBV-HCC. The following five mutational sites were identified as statistically significant independent predictors of HBV-HCC survival: 1915, 2134, 2176, 2221, 2260. The mutational site of 1979 and 2245 were identified for the association with survival at a borderline significance level.
Conclusions:The portal vein thrombosis, tumor TNM classification, size and seven mutational sites in the PreC/C region were identified as independent predictors of postoperative survival in HCC patients.
