The effects of macrophages with high expression of TL1A on activation and proliferation of hepatic stellate cells in vitro
10.3760/cma.j.issn.1007-3418.2018.05.007
- VernacularTitle: 体外高表达肿瘤坏死样因子配体1A的巨噬细胞对肝星状细胞活化与增殖的影响
- Author:
Yuxin LUO
1
;
Jinbo GUO
;
Fengrong YIN
;
Xiaoxia HUO
;
Libo ZHENG
;
Hong ZHANG
;
Xiaolan ZHANG
Author Information
1. Department of Gastroentology, the Second Hospital of Hebei Medical University, Shijiazhuang 050035, China
- Publication Type:Journal Article
- Keywords:
Macrophages;
Hepatic stellate cells;
Tumor necrosis factor-like ligand 1 aberrance;
Interleukin-1β
;
Platelet-derived growth factor-BB
- From:
Chinese Journal of Hepatology
2018;26(5):347-352
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of macrophages with high expression of TL1A on the activation and proliferation of HSCs in vitro.
Methods:The Bone marrow-derived macrophages (BMMs) and peritoneal macrophages (PMs) from wild type (WT) and myeloid-overexpressed TL1A transgenic mice were isolated, differentiated and activated. HSCs were harvested from activated macrophages culture supernatant (CM). HSCs were detected by immunofluorescence and real-time Q-PCR. And the proliferation was detected by CCK-8 and BrdU assay kit. The levels of IL-1β and PDGF-BB in macrophage culture supernatants were determined by enzyme-linked immunosorbent assay (ELISA).
Results:BMMs-derived CM-intervention HSCs were used to detect the expression of α-smooth muscle actin (α-SMA) on the 2nd, 4th and 6th day respectively by immunofluorescence method. There was no significant difference between the two groups on the 2 nd and the 6th day, P > 0.05; On day 4, the CM/Tg group was significantly higher than that of CM/WT group, P < 0.01; the results of CMs derived from PMs were consistent with the above trend. The expression of α-SMA mRNA on the 2nd, 4th and 6th day was detected by real-time Q-PCR method using BM-derived CMs. No significant difference was found between the groups on the 2nd day (P > 0.05).α-SMA mRNA increased further on the 4th and 6th day, and the level of CM/Tg in CM/Tg group was significantly higher than that in CM/WT group (P < 0.05). The detection results of CMs derived from PMs were consistent with the above trend. The results of CCK-8 assay and BrdU assay showed that the proliferation rate of HSCs in CM Tg group was significantly higher than that in CM/WT group (P < 0.01). The CMs derived from PMs were used to interfere with HSCs. And the results were consistent with the above trend. For BMMs, the levels of IL-1β and PDGF-BB in the lipopolysaccharide (LPS) + IFNγ/Tg culture supernatant were significantly higher than those in the LPS+IFNγ/WT group (P < 0.01). For the culture supernatants of PMs Liquid test results consistent with the above trend.
Conclusion:Macrophages with high expression of TL1A could enhance the activation and proliferation of HSCs by increasing the secretion of IL-1β and PDGF-BB.
